Monorail/Foxa2 regulates floorplate differentiation and specification of oligodendrocytes, serotonergic raphé neurones and cranial motoneurones. - Archive ouverte HAL Access content directly
Journal Articles Development (Cambridge, England) Year : 2005

Monorail/Foxa2 regulates floorplate differentiation and specification of oligodendrocytes, serotonergic raphé neurones and cranial motoneurones.

Will H Norton
  • Function : Author
Maryam Mangoli
  • Function : Author
Zsolt Lele
  • Function : Author
Hans-Martin Pogoda
  • Function : Author
Brianne Diamond
  • Function : Author
Sara Mercurio
  • Function : Author
Claire Russell
  • Function : Author
Hiroki Teraoka
  • Function : Author
Heather L Stickney
  • Function : Author
Gerd-Jörg Rauch
  • Function : Author
Carl-Philipp Heisenberg
  • Function : Author
Corinne Houart
  • Function : Author
Thomas F Schilling
  • Function : Author
Hans-Georg Frohnhoefer
  • Function : Author
Carl J Neumann
  • Function : Author
R Mark Gardiner
  • Function : Author
Uwe Strähle
  • Function : Author
Robert Geisler
  • Function : Author
Michelle Rees
  • Function : Author
William S Talbot
  • Function : Author
Stephen W Wilson
  • Function : Author

Abstract

In this study, we elucidate the roles of the winged-helix transcription factor Foxa2 in ventral CNS development in zebrafish. Through cloning of monorail (mol), which we find encodes the transcription factor Foxa2, and phenotypic analysis of mol-/- embryos, we show that floorplate is induced in the absence of Foxa2 function but fails to further differentiate. In mol-/- mutants, expression of Foxa and Hh family genes is not maintained in floorplate cells and lateral expansion of the floorplate fails to occur. Our results suggest that this is due to defects both in the regulation of Hh activity in medial floorplate cells as well as cell-autonomous requirements for Foxa2 in the prospective laterally positioned floorplate cells themselves. Foxa2 is also required for induction and/or patterning of several distinct cell types in the ventral CNS. Serotonergic neurones of the raphenucleus and the trochlear motor nucleus are absent in mol-/- embryos, and oculomotor and facial motoneurones ectopically occupy ventral CNS midline positions in the midbrain and hindbrain. There is also a severe reduction of prospective oligodendrocytes in the midbrain and hindbrain. Finally, in the absence of Foxa2, at least two likely Hh pathway target genes are ectopically expressed in more dorsal regions of the midbrain and hindbrain ventricular neuroepithelium, raising the possibility that Foxa2 activity may normally be required to limit the range of action of secreted Hh proteins.

Dates and versions

hal-00187711 , version 1 (15-11-2007)

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Will H Norton, Maryam Mangoli, Zsolt Lele, Hans-Martin Pogoda, Brianne Diamond, et al.. Monorail/Foxa2 regulates floorplate differentiation and specification of oligodendrocytes, serotonergic raphé neurones and cranial motoneurones.. Development (Cambridge, England), 2005, 132 (4), pp.645-58. ⟨10.1242/dev.01611⟩. ⟨hal-00187711⟩
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