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Article Dans Une Revue Diamond and Related Materials Année : 2007

Covalent linking of peptides onto oxygen-terminated boron-doped diamond surfaces.

Résumé

This paper reports on the formation of semicarbazide boron-doped diamond surfaces and their use for the prepn. of peptide microarrays through site-specific a-oxo semicarbazone ligation. Hydrogen-terminated diamond substrates were first photochem. oxidized and the resulting surface hydroxyl groups were coupled with (aminopropyl)triethoxysilane to yield amine-terminated surfaces. Chem. reaction of the terminal amino groups with triphosgene and Fmoc-protected hydrazine, followed by a deprotection step led to the formation of a semicarbazide termination. Peptides bearing a glyoxylyl group were linked to the semicarbazide-terminated BDD surfaces through site-specific ligation. XPS and fluorescence measurements were used to characterize the resulting surfaces.

Dates et versions

hal-00179751 , version 1 (16-10-2007)

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Citer

Leon Gengembre, Rabah Boukherroub, Yannick Coffinier, Sabine Szunerits, Bernadette Marcus, et al.. Covalent linking of peptides onto oxygen-terminated boron-doped diamond surfaces.. Diamond and Related Materials, 2007, 16 (4-7), pp.892-898. ⟨10.1016/j.diamond.2006.12.058⟩. ⟨hal-00179751⟩
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