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Article Dans Une Revue Biotechnology Journal Année : 2007

Identification of intracellular targets of small molecular weight chemical compounds using affinity chromatography.

Résumé

Efforts to characterize small molecular weight chemical inhibitors of pharmacological interest tend to identify molecules with high efficiency and selectivity, to meet the two criteria required for the clinical development of a drug: efficacy and harmlessness. Drug candidates are expected to inhibit efficiently the target they have been optimized against (for example, a particular type of protein kinase). These hits are also designed to not interfere (or as little as possible) with the activity of other cellular enzymes/proteins to reduce undesired side effects. Here we discuss the use of immobilized drugs as affinity chromatography matrices to purify and identify their bona fide intracellular targets. This method not only allows the systematic investigation of the selectivity of pharmacological compounds but also the anticipation of their putative adverse effects.

Dates et versions

hal-00169462 , version 1 (04-09-2007)

Identifiants

Citer

Damien Guiffant, Déborah Tribouillard, Fabienne Gug, Hervé Galons, Laurent Meijer, et al.. Identification of intracellular targets of small molecular weight chemical compounds using affinity chromatography.. Biotechnology Journal, 2007, 2 (1), pp.68-75. ⟨10.1002/biot.200600223⟩. ⟨hal-00169462⟩
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