PDNA as building blocks for membrane-guided self-assemblies. - Archive ouverte HAL
Article Dans Une Revue Biochemical Society Transactions Année : 2007

PDNA as building blocks for membrane-guided self-assemblies.

Résumé

Different semi-synthetic PDNAs (protein-DNA complexes), which encompass a protein core engineered from the cytochrome b(5) scaffold, an embedded tuneable redox cofactor, a synthetic linker and a large oligonucleotide, were designed, synthesized and purified to homogeneity. These building blocks can be reversibly attached to Ni-DOGS [1,2-dioleoyl-sn-glycero-3-[N(5-amino-1-carboxypentyl)iminodiacetic acid]succinyl]-doped supported membranes through a metal chelate bridge with the protein part and be polymerized in a fully controllable manner using a solid-phase synthesis strategy and a stepwise addition of suitable complementary oligonucleotides. The resulting structures could recreate a large range of regular distribution of patterned redox and absorbing centres separated by fully tuneable distances and geometry. Kinetic parameters for the self-assembly of building blocks were determined using SPRI (surface plasmon resonance imagery). Structures of resulting nano-objects were characterized using gel electrophoresis and single molecule approaches following decoration of assemblies with quantum dots.
Fichier non déposé

Dates et versions

hal-00167420 , version 1 (20-08-2007)

Identifiants

Citer

D. Pompon, A. Laisné. PDNA as building blocks for membrane-guided self-assemblies.. Biochemical Society Transactions, 2007, 35 (Pt 3), pp.495-7. ⟨10.1042/BST0350495⟩. ⟨hal-00167420⟩
9 Consultations
0 Téléchargements

Altmetric

Partager

More