IL-13 induces expression of CD36 in human monocytes through PPARgamma activation. - Archive ouverte HAL Access content directly
Journal Articles European Journal of Immunology Year : 2007

IL-13 induces expression of CD36 in human monocytes through PPARgamma activation.

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Abstract

The class B scavenger receptor CD36 is a component of the pattern recognition receptors on monocytes that recognizes a variety of molecules. CD36 expression in monocytes depends on exposure to soluble mediators. We demonstrate here that CD36 expression is induced in human monocytes following exposure to IL-13, a Th2 cytokine, via the peroxisome proliferator-activated receptor (PPAR)gamma pathway. Induction of CD36 protein was paralleled by an increase in CD36 mRNA. The PPARgamma pathway was demonstrated using transfection of a PPARgamma expression plasmid into the murine macrophage cell line RAW264.7, expressing very low levels of PPARgamma, and in peritoneal macrophages from PPARgamma-conditional null mice. We also show that CD36 induction by IL-13 via PPARgamma is dependent on phospholipase A2 activation and that IL-13 induces the production of endogenous 15-deoxy-Delta12,14-prostaglandin J2, an endogenous PPARgamma ligand, and its nuclear localization in human monocytes. Finally, we demonstrate that CD36 and PPARgamma are involved in IL-13-mediated phagocytosis of Plasmodium falciparum-parasitized erythrocytes. These results reveal a novel role for PPARgamma in the alternative activation of monocytes by IL-13, suggesting that endogenous PPARgamma ligands, produced by phospholipase A2 activation, could contribute to the biochemical and cellular functions of CD36.
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hal-00166681 , version 1 (08-08-2007)

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Antoine Berry, Patricia Balard, Agnès Coste, David Olagnier, Céline Lagane, et al.. IL-13 induces expression of CD36 in human monocytes through PPARgamma activation.. European Journal of Immunology, 2007, 37 (6), pp.1642-52. ⟨10.1002/eji.200636625⟩. ⟨hal-00166681⟩
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