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Article Dans Une Revue Molecular and General Genetics MGG Année : 2000

Lupine leghemoglobin I: expression in transgenic Lotus and tobacco tissues.

Résumé

The proximal parts of the promoters of the genes for symbiotic-type hemoglobins are generally conserved, but the promoter of the lbI gene of lupine (LulbI) shows some unusual structural features. It lacks typical organ-specific elements characteristic of all the leghemoglobin gene promoters described thus far. We have analysed its functional activity in transgenic Lotus corniculatus. A fusion construct between the lbI promoter and the GUS reporter gene was expressed mainly in the central zone of the root nodule, but the product was also detected in the non-nodule root zone and in roots in tissue culture. In roots of transgenic tobacco, the activity of the promoter was only 24% lower than in Lotus nodules. LulbI promoter activity was also detected in tobacco leaves. Lupine hemoglobin I has a higher sequence identity to symbiotic-type hemoglobins and thus it groups within the "Class II" hemoglobins.
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Dates et versions

hal-00165544 , version 1 (26-07-2007)

Identifiants

  • HAL Id : hal-00165544 , version 1
  • PUBMED : 10778735

Citer

P. M. Strózycki, W. M. Kar?owski, Y. Dessaux, A. Petit, A. B. Legocki. Lupine leghemoglobin I: expression in transgenic Lotus and tobacco tissues.. Molecular and General Genetics MGG, 2000, 263 (2), pp.173-82. ⟨hal-00165544⟩
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