High-resolution genetic mapping of the pepper resistancelocus Bs3 governing recognition of the Xanthomonascampestris pv vesicatora AvrBs3 protein - Archive ouverte HAL
Article Dans Une Revue TAG Theoretical and Applied Genetics Année : 2000

High-resolution genetic mapping of the pepper resistancelocus Bs3 governing recognition of the Xanthomonascampestris pv vesicatora AvrBs3 protein

Résumé

The pepper (Capsicum annuum) Bs3 gene confers resistance to Xanthomonas campestris pv vesicatoria strains expressing the avirulence protein AvrBs3. Using amplified fragment length polymorphism (AFLP) and bulked DNA templates from resistant and susceptible plants we identified markers linked to Bs3 and defined a 2.1-cM interval containing the target gene. Bs3-linked AFLP fragments were cloned and conformity of isolated PCR products with the desired markers was determined by hybridisation to membrane-bound AFLP reactions. AFLP markers flanking the target gene were converted into locus-specific PCR-based markers. These markers were employed for the analysis of 790 plants segregating for Bs3, resulting in a linkage map with a genetic resolution of 0.13 cM. Mapping of Bs3-linked markers in tomato placed them to a syntenic interval on tomato chromosome 2.

Dates et versions

hal-00165270 , version 1 (25-07-2007)

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Citer

M. Pierre, L. Noël, T. Lahaye, A. Ballvora, J. Veuskens, et al.. High-resolution genetic mapping of the pepper resistancelocus Bs3 governing recognition of the Xanthomonascampestris pv vesicatora AvrBs3 protein. TAG Theoretical and Applied Genetics, 2000, 101, pp.255-263. ⟨10.1007/s001220051477⟩. ⟨hal-00165270⟩
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