Comparison of circulating antibodies directed against tryptophan derivatives, NO and NO2 modified antigens in Amyotrophic Lateral Sclerosis, Alzheimer syndrome and Multiple Sclerosis. - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2006

Comparison of circulating antibodies directed against tryptophan derivatives, NO and NO2 modified antigens in Amyotrophic Lateral Sclerosis, Alzheimer syndrome and Multiple Sclerosis.

Résumé

The first objective of this study was to identify specific circulating antibodies directed against tryptophan derivatives (TD), NO and NO2- modified amino acid residues in patient sera of amyotrophic lateral sclerosis (ALS), Alzheimer disease (AD), remitting relapsing multiple sclerosis form (RR-MS) and secondary progressive form (SP-MS). The second objective was to determine immunoglobulin classes of identified circulating antibodies. For the antibody evaluation, we synthesized TD conjugates, NO- and NO2-conjugates in order to mimic metabolites induced during the pathogenic processes. The TD antigens were: tryptophan, kynurenin, 3OH-kynurenin, kynurenic acid, picolinic acid, xanthurenic acid, anthranilic acid, 3OH-anthranilic acid, quinolinic acid and quinaldic acid, linked to bovine serum albumin (BSA) using carbodiimide coupling reaction. Then, conjugates were purified and the coupling ratios were evaluated. NO- and NO2-conjugates were the following amino acid residues: nitrotyrosine, tyrosine, phenylalanine, citrulline, tryptophan, asparagine, creatin, cysteine, methionine, histidine, arginine, linked to BSA via glutaraldehyde reaction. After a reduction step and purification, a nitrosylation with NaNO2 was done, except for nitrotyrosine conjugate. All conjugates were coated on well plates for identification of circulating antibodies. High levels of circulating antibodies to TD conjugates, NO- and NO2-conjugates were found in ALS, AD, RR-MS, SP-MS in comparison of healthy control sera, using ELISA assays. Immunodetection of IgG, IgM and IgA classes directed against TD conjugates, NO- and NO2- amino acid residue conjugates was done. These studies shown that: 1) specific circulating antibodies directed against TD, NO- and NO2- modified components were found for each pathology with a specific pattern. The identification of specific antibodies indirectly reveals the stimulation of enzymatic and immunologic mechanism disorders associated with these diseases; 2) specific profiles of immunoglobulins were also observed for each disease. The identification of differential isotype classes to these neoantigens opens new perspectives for a better understanding of these autoimmune and neurodegenerative processes
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Dates et versions

hal-00162051 , version 1 (12-07-2007)

Identifiants

  • HAL Id : hal-00162051 , version 1

Citer

S. Duleu, C. van Der Velden, D. Bodet, F. Poulletier de Gannes, M.P. Dabadie, et al.. Comparison of circulating antibodies directed against tryptophan derivatives, NO and NO2 modified antigens in Amyotrophic Lateral Sclerosis, Alzheimer syndrome and Multiple Sclerosis.. 16th ENS Meeting, May 2006, Lausanne, Switzerland. ⟨hal-00162051⟩
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