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Communication Dans Un Congrès Année : 2006

Potential genotoxic effects of mobile phone signals on the brain: in vitro and in vivo studies

Résumé

Objective. Several in vitro studies have shown that mobile phone signals (in the range of 900 to 2000 MHz) may induce DNA damage. For instance, DNA damage were found in human fibroblasts following continuous or intermittent exposure at 1.2 to 2 W/kg SAR (Diem et al, 2005), and chromosomal damage in human lymphocytes at high SAR (more than 5 W/kg) following continuous exposure (Tice et al, 2002) whereas others researches concluded in the absence of genotoxic effects (Vijayalaxmi et al, 2000; Lagroye et al, 2004; Zeni et al, 2005). There is thus a need for further investigations in order to conclude about RF genotoxic effects. That is why we focused on potential genotoxic effect of GSM-1800 and UMTS signals on nerve cells in vitro and rat brains in vivo. Methods. In vitro, the SH-SY5Y and CHME-5 human nerve cell lines, which are neuroblastoma and microglial cells, respectively, were intermittently (5 min ON/ 10 min OFF) or continuously exposed to a 217 Hz modulated GSM-1800 signal during 1 to 24 hours at the SAR 2 W/kg and assayed using the alkaline comet assay. The SXC1800 exposure system was manufactured by ITIS (Zurich, Switzerland). It consists in two shorted waveguides allowing to run exposed and sham conditions at the same time in the same culture incubator at 37 Celsius degree 5 % CO2. All experiments were performed under blind conditions. Under these conditions, 4-6 independent experiments were performed for each cellular type (minimum 2 Petri dishes by run). In vivo experiments were performed on Sprague-Dawley male rats. The exposure system consisted in a rocket inside which the animal was placed, the UMTS signal at 2000 MHz was applied to the rat heads by the mean of a loop antenna, at 0, 2, and 10 W/kg under acute conditions (2 hours). After the exposure, the animals were immediately sacrificed and the brain was prepared for the alkalin comet assay. Under these conditions, the different groups were constituted of 16 animals. Positive controls were performed using UVB or ethylmethylsulfonate (EMS) under in vitro and in vivo conditions, respectively. At the end of the exposures, cells were removed from their Petri dish by trypsin/EDTA treatment or were extracted from brain homogenate, counted and 5x104 cells were used to detect single DNA breaks. The genotoxic effects were detected using the alkaline comet assay kit (R&D system, Trevigen slides) following the supplier procedure. Under these conditions, 4-6 independent experiments were performed for each cellular type (minimum 2 Petri dishes by run) and. The analysis was done on at least 100 images from two comet slides (one per Petri dish) for each model and exposure condition. Results. The comet analysis was done on at least 100 images from two slides for each model and exposure condition. Analysis of the slides is ongoing. Conclusion. Once the data analysis is completed, ITIS will break the blinding codes, and the results will be presented at the meeting
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Dates et versions

hal-00161893 , version 1 (11-07-2007)

Identifiants

  • HAL Id : hal-00161893 , version 1

Citer

Sébastien Sanchez, F. Poulletier de Gannes, E. Haro, G. Ruffié, I. Lagroye, et al.. Potential genotoxic effects of mobile phone signals on the brain: in vitro and in vivo studies. 28th Annual Meeting of the BEMS, Jun 2006, Cancun, Mexico. ⟨hal-00161893⟩
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