We investigated the distributions and d¹³C values of lipid biomarkers in authigenic methane-related carbonate crusts formed on eastern Mediterranean mud volcanoes.Ahighly diverse suite of microbial membrane lipids was detected. Strongly ¹³C-depleted lipids diagnostic for archaea include isoprenoid glycerol diethers, isoprenoid glycerol dialkyl glycerol tetraethers and several C₂₀-C₃₅ isoprenoid hydrocarbons. Bacterial lipids mainly consist of dialkyl glycerol diethers, branched fatty acids and hopanoids, showing important ¹³C-depletions, albeit less than archaeal lipids. The data provide further evidence for the involvement of archaea and bacteria in the anaerobic oxidation of methane (AOM) and in the sequestration of methane-derived carbon in authigenic carbonate crusts. Varying distributions and isotopic compositions of ¹³C-depleted lipids argue for heterogeneous AOM-related microbial consortia, expanding on existing work at cold seeps. Distinctive biomarker features - and inferred microbial assemblages - are observed in these crusts, in agreement with available phylogenetic data for the samples. Our results support the potential of lipids for distinguishing methanotrophic communities. Despite the observed variances, conservative features in the abundances and isotopic offsets of microbial biomarkers occur, likely reflecting the overall relationships between archaea and bacteria and the nature of carbon flow between them.