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Article Dans Une Revue Cellular and Molecular Life Sciences Année : 2007

Nitric oxide synthase reduces nitrite to NO under anoxia

Anatoly Vanin
  • Fonction : Auteur
L. Bevers
  • Fonction : Auteur
Anny Slama-Schwok
Ernst van Fassen
  • Fonction : Auteur

Résumé

Cultured bEND.3 endothelial cells show a marked increase in NO production when subjected to anoxia, even though the normal arginine pathway of NO formation is blocked due to absence of oxygen. The rate of anoxic NO production exceeds basal unstimulated NO synthesis in normoxic cells. The anoxic release of NO is mediated by endothelial nitric oxide synthase (eNOS), can be abolished by inhibitors of NOS and is accompanied by consumption of intracellular nitrite. The anoxic NO release is unaffected by the xanthine oxidase inhibitor oxypurinol. The phenomenon is attributed to anoxic reduction of intracellular nitrite by eNOS, and its magnitude and duration suggests that the nitrite reductase activity of eNOS is relevant for fast NO delivery in hypoxic vascular tissues.

Dates et versions

hal-00144479 , version 1 (03-05-2007)

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Citer

Anatoly Vanin, L. Bevers, Anny Slama-Schwok, Ernst van Fassen. Nitric oxide synthase reduces nitrite to NO under anoxia. Cellular and Molecular Life Sciences, 2007, 64 (1), pp.96-103. ⟨10.1007/s00018-006-6374-2⟩. ⟨hal-00144479⟩
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