Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Microscopy Research and Technique Année : 2007

Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy

Résumé

The organization of collagen during fibrotic processes is poorly characterized because of the lack of appropriate methodologies. Here we show that multimodal multiphoton microscopy provides novel insights into lung fibrosis. We characterize normal and fibrotic pulmonary tissue in the bleomycin model, and show that second-harmonic generation by fibrillar collagen reveals the micrometer-scale three-dimensional spatial distribution of the fibrosis. We find that combined two-photon excited fluorescence and second-harmonic imaging of unstained lung tissue allows separating the inflammatory and fibrotic steps in this pathology, underlining characteristic features of fibroblastic foci in human Idiopathic Pulmonary Fibrosis samples. Finally, we propose phenomenological scores of lung fibrosis and we show that they unambiguously sort out control and treated mice, with a better sensitivity and reproducibility in the subpleural region. These results should be readily generalized to other organs, as an accurate method to assess extracellular matrix remodeling during fibrosis. Microsc. Res. Tech., 2007. © 2006 Wiley-Liss, Inc.

Dates et versions

hal-00144476 , version 1 (03-05-2007)

Identifiants

Citer

Ana-Maria Pena, Aurelie J Fabre, Delphine Débarre, J. Marchal-Somme, Bruno Crestani, et al.. Three-dimensional investigation and scoring of extracellular matrix remodeling during lung fibrosis using multiphoton microscopy. Microscopy Research and Technique, 2007, 70 (2), pp.162-170. ⟨10.1002/jemt.20400⟩. ⟨hal-00144476⟩
87 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More