In vivo Bioluminescence Imaging of Ca Signalling in the Brain of Drosophila. - Archive ouverte HAL
Article Dans Une Revue PLoS ONE Année : 2007

In vivo Bioluminescence Imaging of Ca Signalling in the Brain of Drosophila.

Résumé

Many different cells' signalling pathways are universally regulated by Ca(2+) concentration [Ca(2+)] rises that have highly variable amplitudes and kinetic properties. Optical imaging can provide the means to characterise both the temporal and spatial aspects of Ca(2+) signals involved in neurophysiological functions. New methods for in vivo imaging of Ca(2+) signalling in the brain of Drosophila are required for probing the different dynamic aspects of this system. In studies here, whole brain Ca(2+) imaging was performed on transgenic flies with targeted expression of the bioluminescent Ca(2+) reporter GFP-aequorin (GA) in different neural structures. A photon counting based technique was used to undertake continuous recordings of cytosolic [Ca(2+)] over hours. Time integrals for reconstructing images and analysis of the data were selected offline according to the signal intensity. This approach allowed a unique Ca(2+) response associated with cholinergic transmission to be identified by whole brain imaging of specific neural structures. Notably, [Ca(2+)] transients in the Mushroom Bodies (MBs) following nicotine stimulation were accompanied by a delayed secondary [Ca(2+)] rise (up to 15 min. later) in the MB lobes. The delayed response was sensitive to thapsigargin, suggesting a role for intra-cellular Ca(2+) stores. Moreover, it was reduced in dunce mutant flies, which are impaired in learning and memory. Bioluminescence imaging is therefore useful for studying Ca(2+) signalling pathways and for functional mapping of neurophysiological processes in the fly brain.

Dates et versions

hal-00140078 , version 1 (04-04-2007)

Identifiants

Citer

Jean-Rene Martin, Kelly L Rogers, Carine Chagneau, Philippe Brûlet. In vivo Bioluminescence Imaging of Ca Signalling in the Brain of Drosophila.. PLoS ONE, 2007, 2, pp.e275. ⟨10.1371/journal.pone.0000275⟩. ⟨hal-00140078⟩
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