<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-00126410</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-03T07:12:38+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="ro">Plasmodium falciparum subtilisin-like protease 2, a merozoite candidate for the merozoite surface protein 1-42 maturase.</title>
            <author role="crp">
              <persName>
                <forename type="first">J. C.</forename>
                <surname>Barale</surname>
              </persName>
              <email type="md5">9ddd3a8baeda0fab0cae9e591b72d5a7</email>
              <email type="domain">pasteur.fr</email>
              <idno type="idhal" notation="numeric">837769</idno>
              <idno type="halauthorid" notation="string">189557-837769</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-1203-0376</idno>
              <idno type="IDREF">https://www.idref.fr/167796399</idno>
              <affiliation ref="#struct-28095"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">T.</forename>
                <surname>Blisnick</surname>
              </persName>
              <email type="md5">1890f722fd12e47554dd50f1824f5987</email>
              <email type="domain">pasteur.fr</email>
              <idno type="idhal" notation="string">thierry-blisnick</idno>
              <idno type="idhal" notation="numeric">746536</idno>
              <idno type="halauthorid" notation="string">189555-746536</idno>
              <affiliation ref="#struct-28095"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">H.</forename>
                <surname>Fujioka</surname>
              </persName>
              <idno type="halauthorid">189561-0</idno>
              <affiliation ref="#struct-28096"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Pedro Maria</forename>
                <surname>Alzari</surname>
              </persName>
              <email type="md5">19e0167f5bdc82ab5a31addeb4366c7e</email>
              <email type="domain">pasteur.fr</email>
              <idno type="idhal" notation="numeric">858583</idno>
              <idno type="halauthorid" notation="string">382015-858583</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-4233-1903</idno>
              <idno type="IDREF">https://www.idref.fr/071021094</idno>
              <affiliation ref="#struct-28098"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">M.</forename>
                <surname>Aikawa</surname>
              </persName>
              <idno type="halauthorid">189569-0</idno>
              <affiliation ref="#struct-28097"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">C.</forename>
                <surname>Braun-Breton</surname>
              </persName>
              <email type="md5">ec697b37bc056f8bbd9e7527b3c6ce37</email>
              <email type="domain">univ-montp2.fr</email>
              <idno type="idhal" notation="numeric">837768</idno>
              <idno type="halauthorid" notation="string">189570-837768</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-3494-788X</idno>
              <idno type="IDREF">https://www.idref.fr/081138016</idno>
              <idno type="VIAF">https://viaf.org/viaf/189569174</idno>
              <idno type="ISNI">http://isni.org/isni/0000000356589267</idno>
              <affiliation ref="#struct-28095"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">G.</forename>
                <surname>Langsley</surname>
              </persName>
              <idno type="halauthorid">189571-0</idno>
              <affiliation ref="#struct-28095"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Jean-Christophe</forename>
                <surname>Barale</surname>
              </persName>
              <email type="md5">9ddd3a8baeda0fab0cae9e591b72d5a7</email>
              <email type="domain">pasteur.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2007-01-24 23:55:37</date>
              <date type="whenModified">2024-09-23 19:35:48</date>
              <date type="whenReleased">2007-01-24 23:55:37</date>
              <date type="whenProduced">1999-05-25</date>
              <ref type="externalLink" target="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC26901"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="116030">
                <persName>
                  <forename>Jean-Christophe</forename>
                  <surname>Barale</surname>
                </persName>
                <email type="md5">9ddd3a8baeda0fab0cae9e591b72d5a7</email>
                <email type="domain">pasteur.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-00126410</idno>
            <idno type="halUri">https://hal.science/hal-00126410</idno>
            <idno type="halBibtex">barale:hal-00126410</idno>
            <idno type="halRefHtml">&lt;i&gt;Proceedings of the National Academy of Sciences of the United States of America&lt;/i&gt;, 1999, 96 (11), pp.6445-50. &lt;a target="_blank" href="https://dx.doi.org/10.1073/pnas.96.11.6445"&gt;&amp;#x27E8;10.1073/pnas.96.11.6445&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Proceedings of the National Academy of Sciences of the United States of America, 1999, 96 (11), pp.6445-50. &amp;#x27E8;10.1073/pnas.96.11.6445&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="PASTEUR">Institut Pasteur</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="RIIP_PARIS">Institut Pasteur de Paris</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="ro">Plasmodium falciparum subtilisin-like protease 2, a merozoite candidate for the merozoite surface protein 1-42 maturase.</title>
                <author role="crp">
                  <persName>
                    <forename type="first">J. C.</forename>
                    <surname>Barale</surname>
                  </persName>
                  <email type="md5">9ddd3a8baeda0fab0cae9e591b72d5a7</email>
                  <email type="domain">pasteur.fr</email>
                  <idno type="idhal" notation="numeric">837769</idno>
                  <idno type="halauthorid" notation="string">189557-837769</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-1203-0376</idno>
                  <idno type="IDREF">https://www.idref.fr/167796399</idno>
                  <affiliation ref="#struct-28095"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">T.</forename>
                    <surname>Blisnick</surname>
                  </persName>
                  <email type="md5">1890f722fd12e47554dd50f1824f5987</email>
                  <email type="domain">pasteur.fr</email>
                  <idno type="idhal" notation="string">thierry-blisnick</idno>
                  <idno type="idhal" notation="numeric">746536</idno>
                  <idno type="halauthorid" notation="string">189555-746536</idno>
                  <affiliation ref="#struct-28095"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">H.</forename>
                    <surname>Fujioka</surname>
                  </persName>
                  <idno type="halauthorid">189561-0</idno>
                  <affiliation ref="#struct-28096"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Pedro Maria</forename>
                    <surname>Alzari</surname>
                  </persName>
                  <email type="md5">19e0167f5bdc82ab5a31addeb4366c7e</email>
                  <email type="domain">pasteur.fr</email>
                  <idno type="idhal" notation="numeric">858583</idno>
                  <idno type="halauthorid" notation="string">382015-858583</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-4233-1903</idno>
                  <idno type="IDREF">https://www.idref.fr/071021094</idno>
                  <affiliation ref="#struct-28098"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">M.</forename>
                    <surname>Aikawa</surname>
                  </persName>
                  <idno type="halauthorid">189569-0</idno>
                  <affiliation ref="#struct-28097"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">C.</forename>
                    <surname>Braun-Breton</surname>
                  </persName>
                  <email type="md5">ec697b37bc056f8bbd9e7527b3c6ce37</email>
                  <email type="domain">univ-montp2.fr</email>
                  <idno type="idhal" notation="numeric">837768</idno>
                  <idno type="halauthorid" notation="string">189570-837768</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-3494-788X</idno>
                  <idno type="IDREF">https://www.idref.fr/081138016</idno>
                  <idno type="VIAF">https://viaf.org/viaf/189569174</idno>
                  <idno type="ISNI">http://isni.org/isni/0000000356589267</idno>
                  <affiliation ref="#struct-28095"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">G.</forename>
                    <surname>Langsley</surname>
                  </persName>
                  <idno type="halauthorid">189571-0</idno>
                  <affiliation ref="#struct-28095"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">7969</idno>
                <idno type="issn">0027-8424</idno>
                <idno type="eissn">1091-6490</idno>
                <title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
                <imprint>
                  <publisher>National Academy of Sciences</publisher>
                  <biblScope unit="volume">96</biblScope>
                  <biblScope unit="issue">11</biblScope>
                  <biblScope unit="pp">6445-50</biblScope>
                  <date type="datePub">1999-05-25</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1073/pnas.96.11.6445</idno>
              <idno type="pubmed">10339607</idno>
              <idno type="pubmedcentral">PMC26901</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="mesh">Amino Acid Sequence</classCode>
              <classCode scheme="mesh">Animals</classCode>
              <classCode scheme="mesh">Merozoite Surface Protein 1</classCode>
              <classCode scheme="mesh">Molecular Sequence Data</classCode>
              <classCode scheme="mesh">Phylogeny</classCode>
              <classCode scheme="mesh">Plasmodium falciparum</classCode>
              <classCode scheme="mesh">Recombinant Fusion Proteins</classCode>
              <classCode scheme="mesh">Sequence Alignment</classCode>
              <classCode scheme="mesh">Sequence Homology, Amino Acid</classCode>
              <classCode scheme="mesh">Subtilisins</classCode>
              <classCode scheme="mesh">Binding Sites</classCode>
              <classCode scheme="mesh">Catalytic Domain</classCode>
              <classCode scheme="mesh">Erythrocytes</classCode>
              <classCode scheme="mesh">Humans</classCode>
              <classCode scheme="halDomain" n="sdv.mp.par">Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>The process of human erythrocyte invasion by Plasmodium falciparum parasites involves a calcium-dependent serine protease with properties consistent with a subtilisin-like activity. This enzyme achieves the last crucial maturation step of merozoite surface protein 1 (MSP1) necessary for parasite entry into the host erythrocyte. In eukaryotic cells, such processing steps are performed by subtilisin-like maturases, known as proprotein convertases. In an attempt to characterize the MSP1 maturase, we have identified a gene that encodes a P. falciparum subtilisin-like protease (PfSUB2) whose deduced active site sequence resembles more bacterial subtilisins. Therefore, we propose that PfSUB2 belongs to a subclass of eukaryotic subtilisins different from proprotein convertases. Pfsub2 is expressed during merozoite differentiation and encodes an integral membrane protein localized in the merozoite dense granules, a secretory organelle whose contents are believed to participate in a late step of the erythrocyte invasion. PfSUB2's subcellular localization, together with its predicted enzymatic properties, leads us to propose that PfSUB2 could be responsible for the late MSP1 maturation step and thus is an attractive target for the development of new antimalarial drugs.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-28095" status="OLD">
          <idno type="RNSR">201021924S</idno>
          <orgName>Biologie des Interactions Hôte-Parasite</orgName>
          <date type="start">2010-01-01</date>
          <date type="end">2014-12-31</date>
          <desc>
            <address>
              <addrLine>25-28 rue du Docteur Roux F-75724 Paris Cedex 15</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-300027" type="direct"/>
            <relation name="URA2581" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-28096" status="INCOMING">
          <orgName>Institute of Pathology</orgName>
          <desc>
            <address>
              <addrLine>Cleveland, OH 44106</addrLine>
              <country key="US"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-365163" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-28098" status="OLD">
          <orgName>Biochimie Structurale</orgName>
          <desc>
            <address>
              <addrLine>28 Rue du Docteur Roux 75724 Paris Cedex 15</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-300027" type="direct"/>
            <relation name="URA2185" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-28097" status="INCOMING">
          <orgName>The Institute of Medical Sciences</orgName>
          <desc>
            <address>
              <addrLine>Kanagawa, Japan 259-1193</addrLine>
              <country key="JP"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-365890" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300027" status="VALID">
          <idno type="IdRef">027936643</idno>
          <idno type="ISNI">0000 0001 2353 6535</idno>
          <idno type="ROR">https://ror.org/0495fxg12</idno>
          <orgName>Institut Pasteur [Paris]</orgName>
          <orgName type="acronym">IP</orgName>
          <date type="start">1887-06-04</date>
          <desc>
            <address>
              <addrLine>25-28, rue du docteur Roux, 75724 Paris cedex 15</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.pasteur.fr</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-365163" status="VALID">
          <idno type="ROR">https://ror.org/051fd9666</idno>
          <orgName>Case Western Reserve University [Cleveland]</orgName>
          <desc>
            <address>
              <addrLine>10900 Euclid Ave., Cleveland, Ohio 44106</addrLine>
              <country key="US"/>
            </address>
            <ref type="url">http://www.case.edu/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-365890" status="VALID">
          <orgName>Tokai University</orgName>
          <date type="start">1942</date>
          <desc>
            <address>
              <country key="JP"/>
            </address>
            <ref type="url">https://www.u-tokai.ac.jp/english/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>