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Article Dans Une Revue Journal of Virology Année : 2006

Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus.

Yves Rouillé
Francois Helle
Cécile Voisset
Sandrine Belouzard
Geert Maertens
  • Fonction : Auteur

Résumé

Due to the recent development of a cell culture model, hepatitis C virus (HCV) can be efficiently propagated in cell culture. This allowed us to reinvestigate the subcellular localization of HCV structural proteins in the context of an infectious cycle. In agreement with previous reports, confocal immunofluorescence analysis of the subcellular localization of HCV structural proteins indicated that, in infected cells, the glycoprotein heterodimer is retained in the endoplasmic reticulum. However, in contrast to other studies, the glycoprotein heterodimer did not accumulate in other intracellular compartments or at the plasma membrane. As previously reported, an association between the capsid protein and lipid droplets was also observed. In addition, a fraction of labeling was consistent with the capsid protein being localized in a membranous compartment that is associated with the lipid droplets. However, in contrast to previous reports, the capsid protein was not found in the nucleus or in association with mitochondria or other well-defined intracellular compartments. Surprisingly, no colocalization was observed between the glycoprotein heterodimer and the capsid protein in infected cells. Electron microscopy analyses allowed us to identify a membrane alteration similar to the previously reported "membranous web." However, no virus-like particles were found in this type of structure. In addition, dense elements compatible with the size and shape of a viral particle were seldom observed in infected cells. In conclusion, the cell culture system for HCV allowed us for the first time to characterize the subcellular localization of HCV structural proteins in the context an infectious cycle.

Domaines

Virologie

Dates et versions

hal-00105500 , version 1 (11-10-2006)

Identifiants

Citer

Yves Rouillé, Francois Helle, David Delgrange, Philippe Roingeard, Cécile Voisset, et al.. Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus.. Journal of Virology, 2006, 80, pp.2832-2841. ⟨10.1128/JVI.80.6.2832-2841.2006⟩. ⟨hal-00105500⟩
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