Article Dans Une Revue Biochemical and Biophysical Research Communications Année : 2005

Identification of SepCRP analogues in the cuttlefish Sepia officinalis: a novel family of ovarian regulatory peptides

B. Bernay
  • Fonction : Auteur
M. Baudy-Floc'H
  • Fonction : Auteur
J. Henry

Résumé

In the cuttlefish, Sepia officinalis, the ovary appears to be one of the main sources of regulatory peptides involved in the successive steps of egg-laying. Following the identification of the SepCRP-1, which is a peptide extracted from ovary and involved in egg capsule secretion, investigations were focused on the identification of related peptides. Seven related-Sepia Capsule Releasing Peptides (R-SepCRPs) were identified by means of mass spectrometry and characterized using MS/MS spectra and/or Edman degradation. Finally, primary structures were verified by the comparison of MS/MS spectra from endogenic and synthetic peptides. This new ovarian peptide family exhibits a conserved SLXKD tag involved in the biological activity. LC-MS/MS screening clearly demonstrates that R-SepCRPs are restricted to the female genital tract. Expressed during vitellogenesis, they are released by vitellogenic follicles and full-grown oocytes (FGO) in the genital coelom. Biological activities suggest that R-SepCRPs would be responsible for the storage of FGO before mating and would take part in the mechanical secretion of egg capsule products, as previously described for SepCRP-1.

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Dates et versions

hal-00096868 , version 1 (20-09-2006)

Identifiants

  • HAL Id : hal-00096868 , version 1

Citer

B. Bernay, M. Baudy-Floc'H, Bruno Zanuttini, J. Gagnon, J. Henry. Identification of SepCRP analogues in the cuttlefish Sepia officinalis: a novel family of ovarian regulatory peptides. Biochemical and Biophysical Research Communications, 2005, 338, pp.1037. ⟨hal-00096868⟩
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