The aim of this study was to investigate the metabolic and physiological responses to a laboratory-based simulated 30-min individual time-trial (ITT 30 ) in cycling at a self-selected intensity. Twelve experienced triathletes (n = 4 women) performed a progressive incremental exercise test on a cycle ergometer to determine .VO2max (52 +/- 5 ml x min -1 x kg -1), maximum power output (300 +/- 12 W), and the second ventilatory threshold. Then, the subjects completed an ITT30 at self-selected work intensity on a stationary ergometer equipped with the SRM Training System. In all subjects, during the ITT30, heart rate and minute ventilation increased (p < 0.05) progressively whereas oxygen consumption and power output remained unchanged. Triathletes rode at consistent pacing corresponding to their highest steady state of blood lactate concentration that increased by no more than 1.0 mmol x l -1 during the final 20-min of ITT30. The self-selected intensity of triathletes during ITT30 represented 88 +/- 5 % (mean +/- SD) of .VO2max and was not significantly different to the energy demand corresponding to the second ventilatory threshold (84 +/- 5 % of .VO2max). Our data suggest that ITT 30 at a self-selected intensity is a good predictor of individual endurance capacity and may be used to estimate racing pace for training purposes. This performance test for the identification of the exercise intensity that demarcate "steady state" is less troublesome than some of the traditional methods, limiting testing to a single session.