<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of cea-02100032</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-19T11:09:01+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli</title>
            <author role="crp">
              <persName>
                <forename type="first">Hervé</forename>
                <surname>Nozach</surname>
              </persName>
              <email type="md5">04dca7df6759718121278af0c8dd4220</email>
              <email type="domain">cea.fr</email>
              <idno type="idhal" notation="numeric">1045759</idno>
              <idno type="halauthorid" notation="string">1125281-1045759</idno>
              <affiliation ref="#struct-40493"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Carole</forename>
                <surname>Fruchart-Gaillard</surname>
              </persName>
              <idno type="halauthorid">592031-0</idno>
              <affiliation ref="#struct-40493"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">François</forename>
                <surname>Fenaille</surname>
              </persName>
              <email type="md5">27875bb1aab1edff8ace82d7a3eca16a</email>
              <email type="domain">cea.fr</email>
              <idno type="idhal" notation="string">francois-fenaille</idno>
              <idno type="idhal" notation="numeric">1136732</idno>
              <idno type="halauthorid" notation="string">1031389-1136732</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-6787-4149</idno>
              <idno type="IDREF">https://www.idref.fr/089320964</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=Y6Qz9wYAAAAJ</idno>
              <idno type="VIAF">https://viaf.org/viaf/197148950</idno>
              <idno type="ISNI">http://isni.org/isni/000000035748802X</idno>
              <affiliation ref="#struct-40494"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Fabrice</forename>
                <surname>Beau</surname>
              </persName>
              <idno type="halauthorid">240846-0</idno>
              <affiliation ref="#struct-40451"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Oscar</forename>
                <surname>Pereira Ramos</surname>
              </persName>
              <email type="md5">0c22aa623a9d2b9c3fb6c257f06d3afb</email>
              <email type="domain">cea.fr</email>
              <idno type="idhal" notation="string">oscar-ramos</idno>
              <idno type="idhal" notation="numeric">1339314</idno>
              <idno type="halauthorid" notation="string">1564671-1339314</idno>
              <affiliation ref="#struct-40493"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Badreddine</forename>
                <surname>Douzi</surname>
              </persName>
              <email type="md5">51de159256ddf60780666ad1dc2b163f</email>
              <email type="domain">inrae.fr</email>
              <idno type="idhal" notation="string">badreddine-douzi</idno>
              <idno type="idhal" notation="numeric">174089</idno>
              <idno type="halauthorid" notation="string">12565-174089</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-3140-4713</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=4du3GJwAAAAJ&amp;hl=en</idno>
              <idno type="IDREF">https://www.idref.fr/159182816</idno>
              <affiliation ref="#struct-199349"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Natalie</forename>
                <surname>Saez</surname>
              </persName>
              <idno type="halauthorid">1374117-0</idno>
              <affiliation ref="#struct-199349"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Mireille</forename>
                <surname>Moutiez</surname>
              </persName>
              <email type="md5">3c04c8c8c0e4b66e9c7b1ee9a875816f</email>
              <email type="domain">cea.fr</email>
              <idno type="idhal" notation="string">mireille-moutiez</idno>
              <idno type="idhal" notation="numeric">753790</idno>
              <idno type="halauthorid" notation="string">421257-753790</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/T-7788-2019</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-0064-8291</idno>
              <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/T-7788-2019</idno>
              <affiliation ref="#struct-460033"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Denis</forename>
                <surname>Servent</surname>
              </persName>
              <email type="md5">476e5974e46681378c8db2c5bd41954e</email>
              <email type="domain">cea.fr</email>
              <idno type="idhal" notation="string">denis-servent</idno>
              <idno type="idhal" notation="numeric">174294</idno>
              <idno type="halauthorid" notation="string">5740-174294</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-0774-1691</idno>
              <idno type="IDREF">https://www.idref.fr/137274882</idno>
              <affiliation ref="#struct-40493"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Muriel</forename>
                <surname>Gondry</surname>
              </persName>
              <idno type="halauthorid">139243-0</idno>
              <affiliation ref="#struct-460033"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Robert</forename>
                <surname>Thai</surname>
              </persName>
              <email type="md5">f513bf87a6918c4bfaa8e290c9da41eb</email>
              <email type="domain">cea.fr</email>
              <idno type="idhal" notation="string">robert-thai</idno>
              <idno type="idhal" notation="numeric">171700</idno>
              <idno type="halauthorid" notation="string">1361-171700</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-9438-4225</idno>
              <affiliation ref="#struct-40493"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Philippe</forename>
                <surname>Cuniasse</surname>
              </persName>
              <idno type="halauthorid">663549-0</idno>
              <affiliation ref="#struct-40451"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Renaud</forename>
                <surname>Vincentelli</surname>
              </persName>
              <email type="md5">5b4065f371b4e8540164195e5a2f806f</email>
              <email type="domain">univ-amu.fr</email>
              <idno type="idhal" notation="string">renaud-vincentelli</idno>
              <idno type="idhal" notation="numeric">751725</idno>
              <idno type="halauthorid" notation="string">382415-751725</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-9667-0196</idno>
              <idno type="IDREF">https://www.idref.fr/151900922</idno>
              <affiliation ref="#struct-199349"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Vincent</forename>
                <surname>Dive</surname>
              </persName>
              <idno type="halauthorid">240843-0</idno>
              <affiliation ref="#struct-40451"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Hervé</forename>
                <surname>Nozach</surname>
              </persName>
              <email type="md5">04dca7df6759718121278af0c8dd4220</email>
              <email type="domain">cea.fr</email>
            </editor>
            <funder ref="#projanr-50581"/>
            <funder ref="#projeurop-86048"/>
            <funder>This work was supported by the ANR PFTV, the GIS IBiSA and by INSTRUCT (ESFRI)</funder>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2019-04-15 14:53:58</date>
              <date type="whenModified">2025-08-20 16:02:02</date>
              <date type="whenReleased">2019-04-17 10:51:12</date>
              <date type="whenProduced">2013-03-30</date>
              <date type="whenEndEmbargoed">2019-04-15</date>
              <ref type="file" target="https://cea.hal.science/cea-02100032v1/document">
                <date notBefore="2019-04-15"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://cea.hal.science/cea-02100032v1/file/Nozach-2013-High%20throughput%20scre.pdf" id="file-2100032-2100957">
                <date notBefore="2019-04-15"/>
              </ref>
              <ref type="externalLink" target="https://microbialcellfactories.biomedcentral.com/track/pdf/10.1186/1475-2859-12-37"/>
              <fs>
                <f name="inra_publicVise_local" notation="string" n="SC">
                  <string>inra_publicVise_local_SC</string>
                </f>
              </fs>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="183755">
                <persName>
                  <forename>Hervé</forename>
                  <surname>Nozach</surname>
                </persName>
                <email type="md5">04dca7df6759718121278af0c8dd4220</email>
                <email type="domain">cea.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">cea-02100032</idno>
            <idno type="halUri">https://cea.hal.science/cea-02100032</idno>
            <idno type="halBibtex">nozach:cea-02100032</idno>
            <idno type="halRefHtml">&lt;i&gt;Microbial Cell Factories&lt;/i&gt;, 2013, 12, &lt;a target="_blank" href="https://dx.doi.org/10.1186/1475-2859-12-37"&gt;&amp;#x27E8;10.1186/1475-2859-12-37&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Microbial Cell Factories, 2013, 12, &amp;#x27E8;10.1186/1475-2859-12-37&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-2100032-2100957"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="CEA">CEA - Commissariat à l'énergie atomique</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-AMU">Aix Marseille Université</idno>
            <idno type="stamp" n="INRA">INRA - Institut national de la recherche agronomique</idno>
            <idno type="stamp" n="OPENAIRE">OpenAIRE</idno>
            <idno type="stamp" n="I2BC">I2BC</idno>
            <idno type="stamp" n="AGREENIUM">Archive ouverte en agrobiosciences</idno>
            <idno type="stamp" n="JOLIOT" corresp="CEA">Institut des Sciences du Vivant Frédéric JOLIOT</idno>
            <idno type="stamp" n="CEA-DRF" corresp="CEA">Direction de Recherche Fondamentale</idno>
            <idno type="stamp" n="DMTS" corresp="JOLIOT">Département Médicaments et Technologies pour la Santé</idno>
            <idno type="stamp" n="SBIGEM" corresp="JOLIOT">Service de Biologie Intégrative et Génétique Moléculaire</idno>
            <idno type="stamp" n="I2BC-SACLAY" corresp="JOLIOT">Institut  de  Biologie  Intégrative  de  la  Cellule  de  Saclay  </idno>
            <idno type="stamp" n="INRAE">Institut National de Recherche en Agriculture, Alimentation et Environnement</idno>
            <idno type="stamp" n="ANR">ANR</idno>
            <idno type="stamp" n="GS-HEALTH-DRUG-SCIENCES">Graduate School Health and Drug Sciences</idno>
            <idno type="stamp" n="AFMB">Architecture et Fonction des Macromolécules Biologiques</idno>
            <idno type="stamp" n="INRAEPACA">INRAE PACA</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">High throughput screening identifies disulfide isomerase DsbC as a very efficient partner for recombinant expression of small disulfide-rich proteins in E. coli</title>
                <author role="crp">
                  <persName>
                    <forename type="first">Hervé</forename>
                    <surname>Nozach</surname>
                  </persName>
                  <email type="md5">04dca7df6759718121278af0c8dd4220</email>
                  <email type="domain">cea.fr</email>
                  <idno type="idhal" notation="numeric">1045759</idno>
                  <idno type="halauthorid" notation="string">1125281-1045759</idno>
                  <affiliation ref="#struct-40493"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Carole</forename>
                    <surname>Fruchart-Gaillard</surname>
                  </persName>
                  <idno type="halauthorid">592031-0</idno>
                  <affiliation ref="#struct-40493"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">François</forename>
                    <surname>Fenaille</surname>
                  </persName>
                  <email type="md5">27875bb1aab1edff8ace82d7a3eca16a</email>
                  <email type="domain">cea.fr</email>
                  <idno type="idhal" notation="string">francois-fenaille</idno>
                  <idno type="idhal" notation="numeric">1136732</idno>
                  <idno type="halauthorid" notation="string">1031389-1136732</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-6787-4149</idno>
                  <idno type="IDREF">https://www.idref.fr/089320964</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=Y6Qz9wYAAAAJ</idno>
                  <idno type="VIAF">https://viaf.org/viaf/197148950</idno>
                  <idno type="ISNI">http://isni.org/isni/000000035748802X</idno>
                  <affiliation ref="#struct-40494"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Fabrice</forename>
                    <surname>Beau</surname>
                  </persName>
                  <idno type="halauthorid">240846-0</idno>
                  <affiliation ref="#struct-40451"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Oscar</forename>
                    <surname>Pereira Ramos</surname>
                  </persName>
                  <email type="md5">0c22aa623a9d2b9c3fb6c257f06d3afb</email>
                  <email type="domain">cea.fr</email>
                  <idno type="idhal" notation="string">oscar-ramos</idno>
                  <idno type="idhal" notation="numeric">1339314</idno>
                  <idno type="halauthorid" notation="string">1564671-1339314</idno>
                  <affiliation ref="#struct-40493"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Badreddine</forename>
                    <surname>Douzi</surname>
                  </persName>
                  <email type="md5">51de159256ddf60780666ad1dc2b163f</email>
                  <email type="domain">inrae.fr</email>
                  <idno type="idhal" notation="string">badreddine-douzi</idno>
                  <idno type="idhal" notation="numeric">174089</idno>
                  <idno type="halauthorid" notation="string">12565-174089</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-3140-4713</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=4du3GJwAAAAJ&amp;hl=en</idno>
                  <idno type="IDREF">https://www.idref.fr/159182816</idno>
                  <affiliation ref="#struct-199349"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Natalie</forename>
                    <surname>Saez</surname>
                  </persName>
                  <idno type="halauthorid">1374117-0</idno>
                  <affiliation ref="#struct-199349"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Mireille</forename>
                    <surname>Moutiez</surname>
                  </persName>
                  <email type="md5">3c04c8c8c0e4b66e9c7b1ee9a875816f</email>
                  <email type="domain">cea.fr</email>
                  <idno type="idhal" notation="string">mireille-moutiez</idno>
                  <idno type="idhal" notation="numeric">753790</idno>
                  <idno type="halauthorid" notation="string">421257-753790</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/T-7788-2019</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-0064-8291</idno>
                  <idno type="RESEARCHERID">http://www.researcherid.com/rid/http://www.researcherid.com/rid/T-7788-2019</idno>
                  <affiliation ref="#struct-460033"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Denis</forename>
                    <surname>Servent</surname>
                  </persName>
                  <email type="md5">476e5974e46681378c8db2c5bd41954e</email>
                  <email type="domain">cea.fr</email>
                  <idno type="idhal" notation="string">denis-servent</idno>
                  <idno type="idhal" notation="numeric">174294</idno>
                  <idno type="halauthorid" notation="string">5740-174294</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-0774-1691</idno>
                  <idno type="IDREF">https://www.idref.fr/137274882</idno>
                  <affiliation ref="#struct-40493"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Muriel</forename>
                    <surname>Gondry</surname>
                  </persName>
                  <idno type="halauthorid">139243-0</idno>
                  <affiliation ref="#struct-460033"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Robert</forename>
                    <surname>Thai</surname>
                  </persName>
                  <email type="md5">f513bf87a6918c4bfaa8e290c9da41eb</email>
                  <email type="domain">cea.fr</email>
                  <idno type="idhal" notation="string">robert-thai</idno>
                  <idno type="idhal" notation="numeric">171700</idno>
                  <idno type="halauthorid" notation="string">1361-171700</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-9438-4225</idno>
                  <affiliation ref="#struct-40493"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Philippe</forename>
                    <surname>Cuniasse</surname>
                  </persName>
                  <idno type="halauthorid">663549-0</idno>
                  <affiliation ref="#struct-40451"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Renaud</forename>
                    <surname>Vincentelli</surname>
                  </persName>
                  <email type="md5">5b4065f371b4e8540164195e5a2f806f</email>
                  <email type="domain">univ-amu.fr</email>
                  <idno type="idhal" notation="string">renaud-vincentelli</idno>
                  <idno type="idhal" notation="numeric">751725</idno>
                  <idno type="halauthorid" notation="string">382415-751725</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-9667-0196</idno>
                  <idno type="IDREF">https://www.idref.fr/151900922</idno>
                  <affiliation ref="#struct-199349"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Vincent</forename>
                    <surname>Dive</surname>
                  </persName>
                  <idno type="halauthorid">240843-0</idno>
                  <affiliation ref="#struct-40451"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">1755</idno>
                <idno type="issn">1475-2859</idno>
                <title level="j">Microbial Cell Factories</title>
                <imprint>
                  <publisher>BioMed Central</publisher>
                  <biblScope unit="volume">12</biblScope>
                  <date type="datePub">2013-03-30</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1186/1475-2859-12-37</idno>
              <idno type="prodinra">480693</idno>
              <idno type="pubmed">23607455</idno>
              <ref target="https://www.ncbi.nlm.nih.gov/pubmed/23607455" type="seeAlso"/>
              <ref type="publisher">http://www.microbialcellfactories.com/content/12/1/37</ref>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">Matrix-assisted laser desorption-ionization</term>
                <term xml:lang="en">Mass</term>
                <term xml:lang="en">Spectrometry</term>
                <term xml:lang="en">Escherichia coli</term>
                <term xml:lang="en">Fusion protein</term>
                <term xml:lang="en">Proteins</term>
                <term xml:lang="en">Disulfide-rich</term>
                <term xml:lang="en">Cytoplasm</term>
                <term xml:lang="en">Disulfide bonds</term>
                <term xml:lang="en">Recombinant expression</term>
                <term xml:lang="en">High throughput screening</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv.bbm">Life Sciences [q-bio]/Biochemistry, Molecular Biology</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>BackgroundDisulfide-rich proteins or DRPs are versatile bioactive compounds that encompass a wide variety of pharmacological, therapeutic, and/or biotechnological applications. Still, the production of DRPs in sufficient quantities is a major bottleneck for their complete structural or functional characterization. Recombinant expression of such small proteins containing multiple disulfide bonds in the bacteria E. coli is considered difficult and general methods and protocols, particularly on a high throughput scale, are limited.ResultsHere we report a high throughput screening approach that allowed the systematic investigation of the solubilizing and folding influence of twelve cytoplasmic partners on 28 DRPs in the strains BL21 (DE3) pLysS, Origami B (DE3) pLysS and SHuffle® T7 Express lysY (1008 conditions). The screening identified the conditions leading to the successful soluble expression of the 28 DRPs selected for the study. Amongst 336 conditions tested per bacterial strain, soluble expression was detected in 196 conditions using the strain BL21 (DE3) pLysS, whereas only 44 and 50 conditions for soluble expression were identified for the strains Origami B (DE3) pLysS and SHuffle® T7 Express lysY respectively. To assess the redox states of the DRPs, the solubility screen was coupled with mass spectrometry (MS) to determine the exact masses of the produced DRPs or fusion proteins. To validate the results obtained at analytical scale, several examples of proteins expressed and purified to a larger scale are presented along with their MS and functional characterization.ConclusionsOur results show that the production of soluble and functional DRPs with cytoplasmic partners is possible in E. coli. In spite of its reducing cytoplasm, BL21 (DE3) pLysS is more efficient than the Origami B (DE3) pLysS and SHuffle® T7 Express lysY trxB - /gor - strains for the production of DRPs in fusion with solubilizing partners. However, our data suggest that oxidation of the proteins occurs ex vivo. Our protocols allow the production of a large diversity of DRPs using DsbC as a fusion partner, leading to pure active DRPs at milligram scale in many cases. These results open up new possibilities for the study and development of DRPs with therapeutic or biotechnological interest whose production was previously a limitation.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="department" xml:id="struct-40493" status="VALID">
          <idno type="RNSR">200722607X</idno>
          <orgName>Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO)</orgName>
          <orgName type="acronym">SIMoS</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <addrLine>Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO Servce d'ingénierie moléculaire des protéines)CEA Saclay 91191 Gif sur Yvette cedex</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation name="DRF/JOLIOT" active="#struct-531263" type="direct"/>
            <relation active="#struct-419361" type="indirect"/>
            <relation active="#struct-487603" type="indirect"/>
            <relation name="DRF" active="#struct-300016" type="indirect"/>
            <relation name="UMR0496" active="#struct-577435" type="indirect"/>
          </listRelation>
        </org>
        <org type="department" xml:id="struct-40494" status="VALID">
          <idno type="RNSR">196317869N</idno>
          <orgName>Service de Pharmacologie et Immunoanalyse</orgName>
          <orgName type="acronym">SPI</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation name="DRF/JOLIOT" active="#struct-531263" type="direct"/>
            <relation active="#struct-419361" type="indirect"/>
            <relation active="#struct-487603" type="indirect"/>
            <relation name="DRF" active="#struct-300016" type="indirect"/>
            <relation name="UMR0496" active="#struct-577435" type="indirect"/>
          </listRelation>
        </org>
        <org type="researchteam" xml:id="struct-40451" status="VALID">
          <orgName>Laboratoire Chimie pour le Vivant, ingénierie moléculaire pour la santé</orgName>
          <orgName type="acronym">LCV</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation name="DRF/JOLIOT" active="#struct-40493" type="direct"/>
            <relation name="DRF/JOLIOT" active="#struct-531263" type="indirect"/>
            <relation active="#struct-419361" type="indirect"/>
            <relation active="#struct-487603" type="indirect"/>
            <relation name="DRF" active="#struct-300016" type="indirect"/>
            <relation name="UMR0496" active="#struct-577435" type="indirect"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-199349" status="OLD">
          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>Faculté des Sciences de Luminy - ESIL 163 avenue de Luminy CP 925 13288 MARSEILLE CEDEX 09</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>
            <relation name="USC1408" active="#struct-92114" type="direct"/>
            <relation name="UMR7257" active="#struct-198056" type="direct"/>
            <relation name="UMR7257" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="researchteam" xml:id="struct-460033" status="VALID">
          <orgName>Enzymologie et Biosynthèse Peptidique Non Ribosomale</orgName>
          <orgName type="acronym">BIOSYN</orgName>
          <desc>
            <address>
              <addrLine>I2BC, Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.i2bc.paris-saclay.fr/spip.php?article1179</ref>
          </desc>
          <listRelation>
            <relation active="#struct-459984" type="direct"/>
            <relation active="#struct-414518" type="indirect"/>
            <relation name="DRF/I2BC" active="#struct-300016" type="indirect"/>
            <relation active="#struct-419361" type="indirect"/>
            <relation name="UMR9198" active="#struct-441569" type="indirect"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-531263" status="VALID">
          <idno type="RNSR">196317869N</idno>
          <orgName>Médicaments et Technologies pour la Santé</orgName>
          <orgName type="acronym">MTS</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <addrLine>CEA- SPI- Bât. 136 Saclay 91191 GIF-SUR-YVETTE</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www6.jouy.inrae.fr/immuno_allergie_alimentaire/Presentation</ref>
          </desc>
          <listRelation>
            <relation active="#struct-419361" type="direct"/>
            <relation active="#struct-487603" type="direct"/>
            <relation name="DRF" active="#struct-300016" type="indirect"/>
            <relation name="UMR0496" active="#struct-577435" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-419361" status="VALID">
          <idno type="IdRef">241345251</idno>
          <idno type="ROR">https://ror.org/03xjwb503</idno>
          <orgName>Université Paris-Saclay</orgName>
          <desc>
            <address>
              <addrLine>Bâtiment Bréguet, 3 Rue Joliot Curie 2e ét, 91190 Gif-sur-Yvette</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.universite-paris-saclay.fr/fr</ref>
          </desc>
        </org>
        <org type="regrouplaboratory" xml:id="struct-487603" status="VALID">
          <idno type="IdRef">262253712</idno>
          <idno type="RNSR">201722498K</idno>
          <idno type="ROR">https://ror.org/016pnwd02</idno>
          <idno type="Wikidata">Q61933008</idno>
          <orgName>Institut des Sciences du Vivant Frédéric JOLIOT</orgName>
          <orgName type="acronym">JOLIOT</orgName>
          <date type="start">2017-01-01</date>
          <desc>
            <address>
              <addrLine>DRFCEAGif-sur-Yvette</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation name="DRF" active="#struct-300016" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-300016" status="VALID">
          <idno type="IdRef">026372061</idno>
          <idno type="ISNI">0000000122998025</idno>
          <idno type="ROR">https://ror.org/00jjx8s55</idno>
          <idno type="Wikidata">Q868550</idno>
          <orgName>Commissariat à l'énergie atomique et aux énergies alternatives</orgName>
          <orgName type="acronym">CEA</orgName>
          <desc>
            <address>
              <addrLine>Centre de SaclayCentre de GrenobleCentre de Cadaracheetc</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.cea.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-577435" status="VALID">
          <idno type="ROR">https://ror.org/003vg9w96</idno>
          <orgName>Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement</orgName>
          <orgName type="acronym">INRAE</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
        <org type="institution" xml:id="struct-92114" status="OLD">
          <idno type="ROR">https://ror.org/01x3gbx83</idno>
          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-198056" status="VALID">
          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <idno type="ROR">https://ror.org/035xkbk20</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>
            <address>
              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="department" xml:id="struct-459984" status="VALID">
          <orgName>Département Microbiologie</orgName>
          <orgName type="acronym">Dpt Microbio</orgName>
          <date type="start">2015-01-01</date>
          <desc>
            <address>
              <addrLine>I2BC, Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.i2bc.paris-saclay.fr/spip.php?article97</ref>
          </desc>
          <listRelation>
            <relation active="#struct-414518" type="direct"/>
            <relation name="DRF/I2BC" active="#struct-300016" type="indirect"/>
            <relation active="#struct-419361" type="indirect"/>
            <relation name="UMR9198" active="#struct-441569" type="indirect"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-414518" status="VALID">
          <idno type="RNSR">201521299R</idno>
          <idno type="ROR">01fftxe08</idno>
          <orgName>Institut de Biologie Intégrative de la Cellule</orgName>
          <orgName type="acronym">I2BC</orgName>
          <date type="start">2015-01-01</date>
          <desc>
            <address>
              <addrLine>Bâtiment 21, 1 avenue de la Terrasse, 91198 Gif/Yvette cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.i2bc.paris-saclay.fr</ref>
          </desc>
          <listRelation>
            <relation name="DRF/I2BC" active="#struct-300016" type="direct"/>
            <relation active="#struct-419361" type="direct"/>
            <relation name="UMR9198" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
      </listOrg>
      <listOrg type="projects">
        <org type="anrProject" xml:id="projanr-50581" status="VALID">
          <idno type="anr">ANR-10-INBS-0005</idno>
          <orgName>FRISBI</orgName>
          <desc>Infrastructure Française pour la Biologie Structurale Intégrée</desc>
          <date type="start">2010</date>
        </org>
        <org type="europeanProject" xml:id="projeurop-86048" status="VALID">
          <idno type="number">278346</idno>
          <idno type="program">FP7-HEALTH-2011-two-stage</idno>
          <idno type="call">FP7-HEALTH-2011-two-stage</idno>
          <orgName>VENOMICS</orgName>
          <desc>High-throughput peptidomics and transcriptomics of animal venoms for discovery of novel therapeutic peptides and innovative drug development</desc>
          <date type="start">2011-01-11</date>
          <date type="end">2015-10-31</date>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>