Preferential effect of synchrotron microbeam radiation therapy on intracerebral 9L gliosarcoma vascular networks. - Grenoble Institut des Neurosciences Accéder directement au contenu
Article Dans Une Revue International Journal of Radiation Oncology, Biology, Physics Année : 2010

Preferential effect of synchrotron microbeam radiation therapy on intracerebral 9L gliosarcoma vascular networks.

Résumé

PURPOSE: Synchrotron microbeam radiation therapy (MRT) relies on spatial fractionation of the incident photon beam into parallel micron-wide beams. Our aim was to analyze the effects of MRT on normal brain and 9L gliosarcoma tissues, particularly on blood vessels. METHODS AND MATERIALS: Responses to MRT (two arrays, one lateral, one anteroposterior (2 × 400 Gy), intersecting orthogonally in the tumor region) were studied during 6 weeks using MRI, immunohistochemistry, and vascular endothelial growth factor Western blot. RESULTS: MRT increased the median survival time of irradiated rats (×3.25), significantly increased blood vessel permeability, and inhibited tumor growth; a cytotoxic effect on 9L cells was detected 5 days after irradiation. Significant decreases in tumoral blood volume fraction and vessel diameter were measured from 8 days after irradiation, due to loss of endothelial cells in tumors as detected by immunochemistry. Edema was observed in the normal brain exposed to both crossfired arrays about 6 weeks after irradiation. This edema was associated with changes in blood vessel morphology and an overexpression of vascular endothelial growth factor. Conversely, vascular parameters and vessel morphology in brain regions exposed to one of the two arrays were not damaged, and there was no loss of vascular endothelia. CONCLUSIONS: We show for the first time that preferential damage of MRT to tumor vessels versus preservation of radioresistant normal brain vessels contributes to the efficient palliation of 9L gliosarcomas in rats. Molecular pathways of repair mechanisms in normal and tumoral vascular networks after MRT may be essential for the improvement of such differential effects on the vasculature.
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Dates et versions

hal-00556537 , version 1 (29-04-2011)

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Audrey Bouchet, Benjamin Lemasson, Géraldine Le Duc, Cécile Maisin, Elke Bräuer-Krisch, et al.. Preferential effect of synchrotron microbeam radiation therapy on intracerebral 9L gliosarcoma vascular networks.. International Journal of Radiation Oncology, Biology, Physics, 2010, 78 (5), pp.1503-12. ⟨10.1016/j.ijrobp.2010.06.021⟩. ⟨hal-00556537⟩
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