smFISH for plants
Résumé
Single molecule fluorescence in situ hybridization (smFISH) is a powerful method for the
visualization and quantification of individual RNA molecules within intact cells. With its ability to probe
gene expression at the single cell and single-molecule level, the technique offers valuable insights into
cellular processes and cell-to-cell heterogeneity. Although widely used in the animal field, its use in
plants has been limited. Here, we present an experimental smFISH workflow that allows researchers to
overcome hybridization and imaging challenges in plants, including sample preparation, probe
hybridization, and signal detection. Overall, this protocol holds great promise for unraveling the
intricacies of gene expression regulation and RNA dynamics at the single-molecule level in whole plants.
Domaines
Chimie
Fichier principal
smFISH in Plants_Methods paper GH_SH[revised].pdf (222.35 Ko)
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