Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons - Oil & Gas Science and Technology – Revue d’IFP Energies nouvelles Accéder directement au contenu
Article Dans Une Revue Oil & Gas Science and Technology - Revue d'IFP Energies nouvelles Année : 2003

Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons

Résumé

In order to evaluate the efficiency of biostimulation of soil contaminated with oil hydrocarbons under sub-Antarctic conditions, a mesocosm study was initiated in May 2001 in the Kerguelen Archipelago (49°21¡¦S, 70°13¡¦E). The effects of temperature and fertilizer addition (Inipol EAP-22, Elf Atochem) on soil bacterial assemblages contaminated with hydrocarbons were studied in 6-l batches of subantarctic soil incubated in the dark. Six different conditions were used at three temperatures (4, 10 and 20°C): control, fertilizer (50 ml), diesel oil (100 ml), diesel oil (100 ml) + fertilizer (50 ml), ¡§Arabian light¡¨ crude oil (100 ml) and crude oil (100 ml) + fertilizer (50 ml). Mesocosms were sampled on a regular basis over a seven-month period. All samples were analyzed for total bacteria, viable heterotrophic assemblages and hydrocarbon-utilising microflora. The results clearly showed a significant response of sub-Antarctic microbial soil communities to hydrocarbon contamination. Large increases in total, heterotrophic and hydrocarbon-utilising bacteria were observed (from less than 5 × 105 MPN g-1 to more than 108 MPN g-1 for hydrocarbon degrading bacteria). Temperature elevation had no significant impact on the total or heterotrophic assemblages but induced a one order of magnitude increase in hydrocarbon-utilising bacteria in contaminated mesocosms. In contrast, fertilizer addition had no clear effect on hydrocarbon-degrading specific bacteria but stimulated heterotrophic growth in diesel oil-contaminated soils.
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Dates et versions

hal-02043879 , version 1 (21-02-2019)

Identifiants

Citer

F. Coulon, D. Delille. Effects of Biostimulation on Growth of Indigenous Bacteria in Sub-Antarctic Soil Contamined with Oil Hydrocarbons. Oil & Gas Science and Technology - Revue d'IFP Energies nouvelles, 2003, 58 (4), pp.469-479. ⟨10.2516/ogst:2003030⟩. ⟨hal-02043879⟩
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