A broad range assay for rapid detection and etiologic characterization of bacterial meningitis: performance testing in samples from sub-Sahara - Département Méthodes quantitatives en santé publique Accéder directement au contenu
Article Dans Une Revue Diagnostic Microbiology and Infectious Disease Année : 2012

A broad range assay for rapid detection and etiologic characterization of bacterial meningitis: performance testing in samples from sub-Sahara

Helen Won
  • Fonction : Auteur
Samuel Yang
  • Fonction : Auteur
Charlotte Gaydos
  • Fonction : Auteur
Justin Hardick
  • Fonction : Auteur
Padmini Ramachandran
  • Fonction : Auteur
Yu-Hsiang Hsieh
  • Fonction : Auteur
Alexander Kecojevic
  • Fonction : Auteur
Tsidi Agbeko Tameklo
  • Fonction : Auteur
Kossi Badziklou
  • Fonction : Auteur
Bradford D Gessner
  • Fonction : Auteur
Richard E Rothman
  • Fonction : Auteur

Résumé

This study aimed to conduct a pilot evaluation of broad-based multiprobe polymerase chain reaction (PCR) in clinical cerebrospinal fluid (CSF) samples compared to local conventional PCR/culture methods used for bacterial meningitis surveillance. A previously described PCR consisting of initial broad-based detection of Eubacteriales by a universal probe, followed by Gram typing, and pathogen-specific probes was designed targeting variable regions of the 16S rRNA gene. The diagnostic performance of the 16S rRNA assay in ""127 CSF samples was evaluated in samples from patients from Togo, Africa, by comparison to conventional PCR/culture methods. Our probes detected Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae. Uniprobe sensitivity and specificity versus conventional PCR were 100% and 54.6%, respectively. Sensitivity and specificity of uniprobe versus culture methods were 96.5% and 52.5%, respectively. Gram-typing probes correctly typed 98.8% (82/83) and pathogen-specific probes identified 96.4% (80/83) of the positives. This broad-based PCR algorithm successfully detected and provided species level information for multiple bacterial meningitis agents in clinical samples.

Dates et versions

hal-03034562 , version 1 (01-12-2020)

Identifiants

Citer

Helen Won, Samuel Yang, Charlotte Gaydos, Justin Hardick, Padmini Ramachandran, et al.. A broad range assay for rapid detection and etiologic characterization of bacterial meningitis: performance testing in samples from sub-Sahara. Diagnostic Microbiology and Infectious Disease, 2012, 74 (1), pp.22-27. ⟨10.1016/j.diagmicrobio.2012.05.015⟩. ⟨hal-03034562⟩
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