Metatranscriptomics reveals the active bacterial and eukaryotic fibrolytic communities in the rumen of dairy cow fed a mixed diet - Archive ouverte HAL Access content directly
Journal Articles Frontiers in Microbiology Year : 2017

Metatranscriptomics reveals the active bacterial and eukaryotic fibrolytic communities in the rumen of dairy cow fed a mixed diet

(1) , (2) , (1) , (1) , (1) , (2) , (3) , (4) , (2) , (1)
1
2
3
4

Abstract

Ruminants have a unique ability to derive energy from the degradation of plant polysaccharides through the activity of the rumen microbiota. Although this process is well studied in vitro, knowledge gaps remain regarding the relative contribution of the microbiota members and enzymes in vivo. The present study used RNA-sequencing to reveal both the expression of genes encoding carbohydrate-active enzymes (CAZymes) by the rumen microbiota of a lactating dairy cow and the microorganisms forming the fiber-degrading community. Functional analysis identified 12,237 CAZymes, accounting for 1% of the transcripts. The CAZyme profile was dominated by families GH94 (cellobiose-phosphorylase), GH13 (amylase), GH43 and GH10 (hemicellulases), GH9 and GH48 (cellulases), PL11 (pectinase) as well as GH2 and GH3 (oligosaccharidases). Our data support the pivotal role of the most characterized fibrolytic bacteria (Prevotella, Ruminocccus and Fibrobacter), and highlight a substantial, although most probably underestimated, contribution of fungi and ciliate protozoa to polysaccharide degradation. Particularly these results may motivate further exploration of the role and the functions of protozoa in the rumen. Moreover, an important part of the fibrolytic bacterial community remains to be characterized since one third of the CAZyme transcripts originated from distantly related strains. These findings are used to highlight limitations of current metatranscriptomics approaches to understand the functional rumen microbial community and opportunities to circumvent them.
Fichier principal
Vignette du fichier
2017_Frontiers_Microbio_Marre_1.pdf (1.41 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-01606055 , version 1 (26-05-2020)

Licence

Attribution - ShareAlike - CC BY 4.0

Identifiers

Cite

Sophie Marre, Nicolas Parisot, Pascale Lepercq, Frédérique Durand, Pascale Mosoni, et al.. Metatranscriptomics reveals the active bacterial and eukaryotic fibrolytic communities in the rumen of dairy cow fed a mixed diet. Frontiers in Microbiology, 2017, 8, pp.67-67. ⟨10.3389/fmicb.2017.00067⟩. ⟨hal-01606055⟩
79 View
82 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More