Extracellular DsbA-insensitive folding of Escherichia coli heat-stable enterotoxin STa in vitro. - Archive ouverte HAL Access content directly
Journal Articles Journal of Biological Chemistry Year : 2000

Extracellular DsbA-insensitive folding of Escherichia coli heat-stable enterotoxin STa in vitro.

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Abstract

To study the folding of human Escherichia coli heat-stable enterotoxin STh, we used the major protein subunit of CS31A fimbriae (ClpG) as a marker of STh secretion and a provider of a signal peptide. We established that STh genetically fused to the N or C terminus of ClpG was able to mobilize ClpG to the culture supernatant while still retaining full enterotoxicity. These features indicate that the STh activity was not altered by the chimeric structure and suggest that spatial conformation of STh in the fusion is close to that of the native toxin, thus permitting recognition and activation of the intestinal STh receptor in vivo. In contrast to other studies, we showed that disulfide bond formation did not occur in the periplasm through the DsbA pathway and that there was no correlation between DsbA and secretion, folding, or activity. This discrepancy was not attributable to the chimeric nature of STh since there was no effect of dsbA or dsbB mutations on secretion and activity of recombinant STh from which ClpG had been deleted. Periplasmic and lysate fractions of dsbA(+) and dsbA(-) cells did not have any STh activity. In addition, the STh chimera was exclusively found in an inactive reduced form intracellularly and in an active oxidized form extracellularly, irrespective of the dsbA background. Subsequently, a time course experiment in regard to the secretion of STh from both dsbA(+) and dsbA(-) cells indicated that the enterotoxin activity (proper folding) in the extracellular milieu increased with time. Overall, these findings provide evidence that STa toxins can be cell-released in an unfolded state before being completely disulfide-bonded outside the cell.
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hal-00529325 , version 1 (01-06-2020)

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I. Batisson, M. Der Vartanian. Extracellular DsbA-insensitive folding of Escherichia coli heat-stable enterotoxin STa in vitro.. Journal of Biological Chemistry, 2000, 275 (14), pp.10582-9. ⟨hal-00529325⟩
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