Metal-chelating activity of soy and pea protein hydrolysates obtained after different enzymatic treatments from protein isolates - LRGP Accéder directement au contenu
Article Dans Une Revue Food Chemistry Année : 2023

Metal-chelating activity of soy and pea protein hydrolysates obtained after different enzymatic treatments from protein isolates

Loïc Stefan

Résumé

Soy and pea proteins are two rich sources of essential amino acids. The hydrolysis of these proteins reveals functional and bioactive properties of the produced small peptide mixtures. In our study, we employed the hydrolysis of soy and pea protein isolates with the endopeptidases Alcalase® and Protamex®, used alone or followed by the exopeptidase Flavourzyme®. The sequential enzyme treatments were the most efficient regarding the degree of hydrolysis. Then, soy and pea protein hydrolysates (SPHs and PPHs, respectively) were ultrafiltrated in order to select peptides of molecular weight ≤ 1 kDa. Whatever the protein source or the hydrolysis treatment, the hydrolysates showed similar molecular weight distributions and amino acid compositions. In addition, all the ultrafiltrated hydrolysates possess metal-chelating activities, as determined by UVspectrophotometry and Surface Plasmon Resonance (SPR). However, the SPR data revealed better chelating affinities in SPHs and PPHs when produced by sequential enzymatic treatment
Fichier principal
Vignette du fichier
El Hajj et al., 2023-HAL.pdf (1.72 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
licence : CC BY - Paternité

Dates et versions

hal-04249612 , version 1 (19-10-2023)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

El Sarah Hajj, Rachel Irankunda, Jairo Andrés Camaño Echavarría, Philippe Arnoux, Cédric Paris, et al.. Metal-chelating activity of soy and pea protein hydrolysates obtained after different enzymatic treatments from protein isolates. Food Chemistry, 2023, 405, pp.134788. ⟨10.1016/j.foodchem.2022.134788⟩. ⟨hal-04249612⟩
185 Consultations
55 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More