Journal Articles International Journal of Bioprinting Year : 2019

Large 3D bioprinted tissue: Heterogeneous perfusion and vascularization

Abstract

Large bioprinted tissues (dm 3 ) are being more and more accessible but their in vitro culture and maturation conditions are still an unknown territory. In the present report, we are presenting a preliminary study of endothelialized large bioprinted tissues (fibroblast and human dermal microvascular endothelial cells) maturation using silicone 3D printed bioreactors. Computational Fluid Dynamics (CFD) simulation was used to link the theoretical culture medium flow path within the large bioprinted tissue with the actual tissue morphology and composition, obtained through histological observations. The obtained results demonstrate the positive impact of using dynamic maturation conditions (300 mL/h culture medium flow rate) on the extracellular matrix production and the conservation of the large bioprinted tissue internal geometry. Clear differences between static and dynamic culture conditions were herein found. Finally, typical microvascular organization, composed of human dermal microvascular endothelial cells organized around an open lumen, were found within the large bioprinted tissue.

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Dates and versions

hal-02133994 , version 1 (12-01-2025)

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Léa Pourchet, Emma Petiot, Céline Loubière, Éric Olmos, Morgan dos Santos, et al.. Large 3D bioprinted tissue: Heterogeneous perfusion and vascularization. International Journal of Bioprinting, 2019, 13, pp.e00039. ⟨10.1016/j.bprint.2018.e00039⟩. ⟨hal-02133994⟩
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