Decoupling filamentous phage uptake and energy of the TolQRA motor in Escherichia coli . - Laboratoire d'ingénierie des systèmes macromoléculaires Accéder directement au contenu
Article Dans Une Revue Journal of Bacteriology Année : 2019

Decoupling filamentous phage uptake and energy of the TolQRA motor in Escherichia coli .

Résumé

Filamentous phages are non-lytic viruses that specifically infect bacteria, establishing a persistent association with their host. The phage particle has no machinery for generating energy and parasitizes its host existing structures in order to cross the bacterial envelope and deliver its genetic material. The import of filamentous phages across the bacterial periplasmic space requires some of the components of a macro-complex of the envelope known as the Tol system. This complex uses the energy provided by the proton-motive force of the inner membrane to perform essential and highly energy-consuming functions of the cell, such as envelope integrity maintenance and cell division. It has been suggested that phages take advantage of pmf-driven conformational changes in the Tol system to transit across the periplasm. However, this hypothesis has not been formally tested. In order to decouple the role of the Tol system in cell physiology and during phage parasitism, we used mutations on conserved essential residues known for inactivating pmf-dependent functions of the Tol system. We identified impaired Tol complexes that remain fully efficient for filamentous phage uptake. We further demonstrate that the TolQ-TolR homologous motor, ExbB-ExbD, normally operating with the TonB protein, is able to promote phage infection along with full length TolA.
Fichier principal
Vignette du fichier
Samire et al. 2019manuscriptHAL-fusionné.pdf (2.2 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02336657 , version 1 (06-02-2020)

Identifiants

Citer

Poutoum Samire, Bastien Serrano, Denis Duché, Emeline Lemarie, Roland Lloubes, et al.. Decoupling filamentous phage uptake and energy of the TolQRA motor in Escherichia coli .. Journal of Bacteriology, 2019, ⟨10.1128/JB.00428-19⟩. ⟨hal-02336657⟩
105 Consultations
70 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More