Organization of collagen fibers and tissue hardening: Markers of fibrotic scarring after spinal cord injury in mice revealed by multiphoton-atomic force microscopy imaging - Laboratoire Charles Coulomb (L2C) Access content directly
Journal Articles Nanomedicine: Nanotechnology, Biology and Medicine Year : 2023

Organization of collagen fibers and tissue hardening: Markers of fibrotic scarring after spinal cord injury in mice revealed by multiphoton-atomic force microscopy imaging

Abstract

Spinal cord injury is a dramatic disease leading to severe motor, sensitive and autonomic impairments. After injury the axonal regeneration is partly inhibited by the glial scar, acting as a physical and chemical barrier. The scarring process involves microglia, astrocytes and extracellular matrix components, such as collagen, con- structing the fibrotic component of the scar. To investigate the role of collagen, we used a multimodal label-free imaging approach combining multiphoton and atomic force microscopy. The second harmonic generation signal exhibited by fibrillar collagen enabled to specifically monitor it as a biomarker of the lesion. An increase in collagen density and the formation of more tortuous fibers over time after injury are observed. Nano-mechanical investigations revealed a noticeable hardening of the injured area, correlated with collagen fibers' formation. These observations indicate the concomitance of important structural and mechanical modifications during the fibrotic scar evolution.
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hal-04195286 , version 1 (04-09-2023)

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Clara Manesco, Oscar Saavedra-Villanueva, Marta Martin, Joshua de Lizaraga, Béla Varga, et al.. Organization of collagen fibers and tissue hardening: Markers of fibrotic scarring after spinal cord injury in mice revealed by multiphoton-atomic force microscopy imaging. Nanomedicine: Nanotechnology, Biology and Medicine, 2023, 53, pp.102699. ⟨10.1016/j.nano.2023.102699⟩. ⟨hal-04195286⟩
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