Relaxation time asymmetry in stator dynamics of the bacterial flagellar motor - Laboratoire Charles Coulomb (L2C) Access content directly
Journal Articles Science Advances Year : 2022

Relaxation time asymmetry in stator dynamics of the bacterial flagellar motor

Maria-José Franco-Oñate
  • Function : Author
  • PersonId : 1132954
Jean-Charles Walter
Jerome Dorignac
  • Function : Author
  • PersonId : 1004442
Frederic Geniet
  • Function : Author
  • PersonId : 1004445
John Palmeri
Andrea Parmeggiani
  • Function : Author
  • PersonId : 1004444
Nils-Ole Walliser
  • Function : Author
  • PersonId : 1024985
Ashley Nord


The bacterial flagellar motor (BFM) is the membrane-embedded rotary molecular motor which turns the flagellum that provides thrust to many bacterial species. This large multimeric complex, composed of a few dozen constituent proteins, has emerged as a hallmark of dynamic subunit exchange. The stator units are inner-membrane ion channels which dynamically bind and unbind to the peptidoglycan at the rotor periphery, consuming the ion motive force (IMF) and applying torque to the rotor when bound. The dynamic exchange is known to be a function of the viscous load on the flagellum, allowing the bacterium to dynamically adapt to its local viscous environment, but the molecular mechanisms of exchange and mechanosensitivity remain to be revealed. Here, by actively perturbing the steady-state stator stoichiometry of individual motors, we reveal a stoichiometry-dependent asymmetry in stator remodeling kinetics. We interrogate the potential effect of next-neighbor interactions and local stator unit depletion and find that neither can explain the observed asymmetry. We then simulate and fit two mechanistically diverse models which recapitulate the asymmetry, finding stator assembly dynamics to be particularly well described by a two-state catch-bond mechanism.
Fichier principal
Vignette du fichier
Asymmetry_Biorxiv.pdf (4.54 Mo) Télécharger le fichier
Asymmetry_SI_Biorxiv.pdf (1.87 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03351194 , version 1 (29-09-2021)



Ruben Perez-Carrasco, Maria-José Franco-Oñate, Jean-Charles Walter, Jerome Dorignac, Frederic Geniet, et al.. Relaxation time asymmetry in stator dynamics of the bacterial flagellar motor. Science Advances , 2022, 8 (12), pp.eabl8112. ⟨10.1126/sciadv.abl8112⟩. ⟨hal-03351194⟩
158 View
84 Download



Gmail Facebook Twitter LinkedIn More