Improving the axial and lateral resolution of three-dimensional fluorescence microscopy using random speckle illuminations - Archive ouverte HAL Access content directly
Journal Articles Journal of the Optical Society of America. A, Optics and image science Year : 2016

Improving the axial and lateral resolution of three-dimensional fluorescence microscopy using random speckle illuminations

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A. Negash
  • Function : Author
Nicolas Sandeau
  • Function : Author
  • PersonId : 833805
Marc Allain
Hugues Giovannini
  • Function : Author
Patrick C. Chaumet
  • Function : Author
Kamal Belkebir
  • Function : Author
  • PersonId : 834041
Anne Sentenac
  • Function : Author

Abstract

We consider a fluorescence microscope in which several three-dimensional images of a sample are recorded for different speckle illuminations. We show, on synthetic data, that by summing the positive deconvolution of each speckle image, one obtains a sample reconstruction with axial and transverse resolutions that compare favorably to that of an ideal confocal microscope.
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Dates and versions

hal-01314573 , version 1 (11-05-2016)

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A. Negash, S. Labouesse, Nicolas Sandeau, Marc Allain, Hugues Giovannini, et al.. Improving the axial and lateral resolution of three-dimensional fluorescence microscopy using random speckle illuminations. Journal of the Optical Society of America. A, Optics and image science, 2016, 33, pp.1089-1094. ⟨10.1364/JOSAA.33.001089⟩. ⟨hal-01314573⟩
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