E5564 inhibits immunosuppressive cytokine IL-10 induction promoted by HIV-1 Tat protein - Infinity Accéder directement au contenu
Article Dans Une Revue Virology Journal Année : 2014

E5564 inhibits immunosuppressive cytokine IL-10 induction promoted by HIV-1 Tat protein

Résumé

Background: In HIV-1 infected patients, production of interleukin-10 (IL-10), a highly immunosuppressive cytokine, is associated with progression of infection toward AIDS. HIV-1 Tat protein, by interacting with TLR4-MD2 at the membrane level, induces IL-10 production by primary human monocytes and macrophages. In the present study we evaluated the effect of the TLR4 antagonist Eritoran tetrasodium (E5564) on HIV-1 Tat-induced IL-10 production. Findings: Here, we confirm that the recombinant HIV-1 Tat protein and the GST-Tat 1-45 fusion protein efficiently stimulate IL-10 production by primary monocytes and macrophages and that this stimulation is inhibited by blocking anti-TLR4 mAbs. We show that a similar inhibition is observed by preincubating the cells with the TLR4 antagonist E5564. Findings:Here, we confirm that the recombinant HIV-1 Tat protein and the GST-Tat 1–45 fusion protein efficiently stimulate IL-10 production by primary monocytes and macrophages and that this stimulation is inhibited by blocking anti-TLR4 mAbs. We show that a similar inhibition is observed by preincubating the cells with the TLR4 antagonist E5564. Conclusion:This study provides compelling data showing for the first time that the TLR4 antagonist E5564 inhibits the immunosuppressive cytokine IL-10 production by primary human monocytes and macrophages incubated in the presence of HIV-1 Tat protein.

Domaines

Virologie
Fichier principal
Vignette du fichier
document(20).pdf (5.07 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02147127 , version 1 (04-06-2019)

Identifiants

Citer

Elmostafa Bahraoui, Laurence Briant, Nathalie Chazal. E5564 inhibits immunosuppressive cytokine IL-10 induction promoted by HIV-1 Tat protein. Virology Journal, 2014, 11, pp.214. ⟨10.1186/s12985-014-0214-z⟩. ⟨hal-02147127⟩
129 Consultations
28 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More