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Article Dans Une Revue Biomedical Physics & Engineering Express Année : 2023

Precision of neuronal localization in 2D cell cultures by using high-performance electropolymerized microelectrode arrays correlated with optical imaging

Luc Buée
Sophie Halliez
Yannick Coffinier

Résumé

Recently, the development of electronic devices to extracellularly record the simultaneous electrical activities of numerous neurons has been blooming, opening new possibilities to interface and decode neuronal activity. In this work, we tested how the use of EDOT electropolymerization to tune post-fabrication materials could optimize the cell/electrode interface of such devices. Our results showed an improved signal-to-noise ratio, better biocompatibility, and a higher number of neurons detected in comparison with gold electrodes. Then, using such enhanced recordings with 2D neuronal cultures combined with fluorescent optical imaging, we checked the extent to which the positions of the recorded neurons could be estimated solely via their extracellular signatures. Our results showed that assuming neurons behave as monopoles, positions could be estimated with a precision of approximately tens of micrometers.
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Origine : Publication financée par une institution
licence : CC BY - Paternité

Dates et versions

hal-03999001 , version 1 (25-04-2023)

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Paternité

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Mahdi Ghazal, Corentin Scholaert, Corentin Dumortier, Camille Lefebvre, Nicolas Barois, et al.. Precision of neuronal localization in 2D cell cultures by using high-performance electropolymerized microelectrode arrays correlated with optical imaging. Biomedical Physics & Engineering Express, 2023, 9 (3), pp.035016. ⟨10.1088/2057-1976/acb93e⟩. ⟨hal-03999001⟩
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