Fluorescence evidence of annexin A6 translocation across membrane in model matrix vesicles during apatite formation - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
Article Dans Une Revue Journal of Extracellular Biology Année : 2022

Fluorescence evidence of annexin A6 translocation across membrane in model matrix vesicles during apatite formation

Liliana Weremiejczyk
  • Fonction : Auteur
Ekeveliny Amabile Veschi
  • Fonction : Auteur
Mayte Bolean
  • Fonction : Auteur
Ana Paula Ramos
  • Fonction : Auteur
Joanna Bandorowicz‐pikula
  • Fonction : Auteur
Slawomir Pikula
  • Fonction : Auteur
Jose Luis Millán
  • Fonction : Auteur
Massimo Bottini
Pietro Ciancaglini
  • Fonction : Auteur
Wei Tao Dou
  • Fonction : Auteur
He Tian
  • Fonction : Auteur
Xiao P He
  • Fonction : Auteur

Résumé

Matrix vesicles (MVs) are 100-300 nm spherical structures released by mineralization competent cells to initiate formation of apatite, the mineral component in bones. Among proteins present in MVs, annexin A6 (AnxA6) is thought to be ubiquitously distributed in the MVs' lumen, on the surface of the internal and external leaflets of the membrane and also inserted in the lipid bilayer. To determine the molecular mechanism(s) that lead to the different locations of AnxA6, we hypothesized the occurrence of a pH drop during the mineralization. Such a change would induce the AnxA6 protonation, which in turn, and because of its isoelectric point of 5.41, would change the protein hydrophobicity facilitating its insertion into the MVs' bilayer. The various distributions of AnxA6 are likely to disturb membrane phospholipid organization. To examine this possibility, we used fluorescein as pH reporter, and established that pH decreased inside MVs during apatite formation. Then, 4-(14-phenyldibenzo[a,c]phenazin-9(14H)-yl)-phenol, a vibration-induced emission fluorescent probe, was used as a reporter of changes in membrane organization occurring with the varying mode of AnxA6 binding. Proteoliposomes containing AnxA6 and 1,2-Dimyristoyl-snglycero-3phosphocholine (DMPC) or 1,2-Dimyristoyl-sn-glycero-3phosphocholine: 1,2-Dipalmitoyl-sn-glycero-3-phosphoserine (DMPC:DPPS 9:1), to mimic the external and internal MV membrane leaflet, respectively, served as biomimetic models to investigate the nature of AnxA6 binding. Addition of Anx6 to DMPC at pH 7.4 and 5.4, or DMPC:DPPS (9:1) at pH 7.4 induced a decrease in membrane fluidity, consistent with AnxA6 interactions with the bilayer surface. In contrast, AnxA6 addition to DMPC:DPPS (9:1) at pH 5.4 increased the fluidity of the membrane. This latest result was interpreted as reflecting the insertion of AnxA6 into the bilayer. Taken together, these findings point to a possible mechanism of AnxA6 translocation in MVs from the surface of the internal leaflet into the phospholipid bilayer stimulated upon acidification of the MVs' lumen during formation of apatite.

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Dates et versions

hal-04835328 , version 1 (13-12-2024)

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Yubo Wang, Liliana Weremiejczyk, Agnieszka Strzelecka‐kiliszek, Ofelia Maniti, Ekeveliny Amabile Veschi, et al.. Fluorescence evidence of annexin A6 translocation across membrane in model matrix vesicles during apatite formation. Journal of Extracellular Biology, 2022, 1, ⟨10.1002/jex2.38⟩. ⟨hal-04835328⟩
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