Extrusion-based 3D printing is a recent and widely used tissue engineering tool that provides precise 3D control of bioinks to create organ-size biomaterial-based objects composed of hierarchically organized cellularized scaffolds. The internal organization of scaffold constituents should mimic the structural anisotropy of targeted tissue to stimulate proliferative cellular behavior during 3D cell culture. Both the choice of polymers constituting the bioink and the topological properties during the extrusion process greatly influence the structural anisotropy and cellular response of tissue engineering constructs. The bioink used in our study was a hydrogel made of three constituents: fibrinogen, alginate and gelatin. These components provide biocompatibility, printability and conservation of 3D shape after printing. The topological properties in flowing polymers are dictated by macromolecule conformation i.e. their orientation and degree of stretch. In this study, we used the micro-macro approach to describe the orientation state of hydrogel macromolecules during the extrusion process, offering a two-scale description of fluid behavior. The goal of our study was to use the Fokker-Planck equation, which describes the evolution of probability distribution function over time, to represent the real state of the constituent population in the representative elementary volume within a hydrogel during extrusion-based 3D printing. Our data suggest that for a tubular nozzle syringe, constituent orientation is driven by a high shear rate, which overcomes the fluid rheological behavior. Also, the interaction coefficient, which represents the microscopic interaction between fluid particles overcomes hydrogel behavior for constituent orientation in the prediction model.