A microfluidic platform to investigate the role of mechanical constraints on tissue reorganization - IBDM - Institut de Biologie du Développement de Marseille Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2023

A microfluidic platform to investigate the role of mechanical constraints on tissue reorganization

Résumé

Mechanical constraints have a high impact on development processes, and there is a need for new tools to investigate the role of mechanosensitive pathways in tissue reorganization during development. We present here experiments where embryonic cell aggregates are aspired through constrictions in microfluidic channels, generating highly heterogeneous flows and high cell deformations that can be imaged using two-photon microscopy. This approach provides a way to measure in situ local viscoelastic properties of 3D tissues and connect them to intracellular and intercellular events such as cell shape changes and cell rearrangements. Perspectives include applications on organoids to investigate and quantify rheological properties of tissues, and to understand how constraints affect development.
Fichier principal
Vignette du fichier
2022.03.16.484527.full.pdf (10.83 Mo) Télécharger le fichier
licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

hal-03873868 , version 1 (22-11-2023)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Sham Tlili, François Graner, Hélène Delanoë-Ayari. A microfluidic platform to investigate the role of mechanical constraints on tissue reorganization. 2023. ⟨hal-03873868⟩
21 Consultations
4 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More