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Article Dans Une Revue eLife Année : 2022

Super-resolution imaging uncovers the nanoscopic segregation of polarity proteins in epithelia

Dominique Massey-Harroche
  • Fonction : Auteur
Fabrice Richard
  • Fonction : Auteur
Jean-Paul Concordet
André Le Bivic
  • Fonction : Auteur

Résumé

Epithelial tissues acquire their integrity and function through the apico-basal polarization of their constituent cells. Proteins of the PAR and Crumbs complexes are pivotal to epithelial polarization, but the mechanistic understanding of polarization is challenging to reach, largely because numerous potential interactions between these proteins and others have been found, without a clear hierarchy in importance. We identify the regionalized and segregated organization of members of the PAR and Crumbs complexes at epithelial apical junctions by imaging endogenous proteins using STED microscopy on Caco-2 cells, and human and murine intestinal samples. Proteins organize in submicrometric clusters, with PAR3 overlapping with the tight junction (TJ) while PALS1-PATJ and aPKC-PAR6β form segregated clusters that are apical of the TJ and present in an alternated pattern related to actin organization. CRB3A is also apical of the TJ and partially overlaps with other polarity proteins. Of the numerous potential interactions identified between polarity proteins, only PALS1-PATJ and aPKC-PAR6β are spatially relevant in the junctional area of mature epithelial cells, simplifying our view of how polarity proteins could cooperate to drive and maintain cell polarity.
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Dates et versions

hal-03852684 , version 1 (15-11-2022)

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Pierre Mangeol, Dominique Massey-Harroche, Fabrice Richard, Jean-Paul Concordet, Pierre-François Lenne, et al.. Super-resolution imaging uncovers the nanoscopic segregation of polarity proteins in epithelia. eLife, 2022, 11, ⟨10.7554/eLife.62087⟩. ⟨hal-03852684⟩
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