Identification of a 130 Kda bcr related gene product. - IBDM - Institut de Biologie du Développement de Marseille Accéder directement au contenu
Article Dans Une Revue Oncogene Année : 1989

Identification of a 130 Kda bcr related gene product.

R Amson
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Adam Telerman
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Résumé

Human chronic myelogenous leukemia is characterized by a reciprocal translocation between chromosomes 9 and 22. This results in the transfer of the c-abl protooncogene from chromosome 9 into the bcr gene on chromosome 22. The purpose of this study was to characterize the bcr and related gene products. Antibodies were raised against a fused trpE-bcr protein induced in a bacterial expression vector. Immunoprecipitation with the monoclonal and polyclonal antibodies of metabolically [35S]methionine labeled leukemic cell lines shows a 210, 160 and 130 Kda protein in Philadelphia positive cells containing the bcr-abl fused transcript. Only the 160 and 130 Kda were present in the Philadelphia negative cells. In vitro kinase assay shows that the 130 Kda protein is a phosphoprotein mainly phosphorylated on serine. Partial proteolysis indicates that the p210 and p130 share common domains. In subcellular fractionation experiments, the p130 is colocalized with the p210 bcr-abl in the cytoplasmic fraction. Together with the mapping of 4 distinct bcr related loci our data suggest that the 130 Kda phosphoprotein belongs to a wider family of bcr related gene products.
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Dates et versions

hal-02412896 , version 1 (16-12-2019)

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  • HAL Id : hal-02412896 , version 1
  • PUBMED : 2494632

Citer

R Amson, C. Marcelle, Adam Telerman. Identification of a 130 Kda bcr related gene product.. Oncogene, 1989, 4 (2), pp.243-7. ⟨hal-02412896⟩

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