AIRR Community Guide to Planning and Performing AIRR-Seq Experiments - Immunologie-Immunopathologie-Immunothérapie Accéder directement au contenu
Chapitre D'ouvrage Année : 2022

AIRR Community Guide to Planning and Performing AIRR-Seq Experiments

Résumé

The development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last decade has witnessed an explosion in protocols, experimental methodologies, and computational tools. In this chapter, we discuss the major considerations in planning a successful AIRR-seq experiment together with basic strategies for controlling and evaluating the outcome of the experiment. Members of the AIRR Community have authored several chapters in this edition, which cover step-by-step instructions to successfully conduct, analyze, and share an AIRR-seq project.
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Dates et versions

hal-03838024 , version 1 (06-04-2024)

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Anne Eugster, Magnolia Bostick, Nidhi Gupta, Encarnita Mariotti-Ferrandiz, Gloria Kraus, et al.. AIRR Community Guide to Planning and Performing AIRR-Seq Experiments. Immunogenetics, 2453, Springer US, pp.261-278, 2022, Methods in Molecular Biology, ⟨10.1007/978-1-0716-2115-8_15⟩. ⟨hal-03838024⟩
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