Low-Dose Pesticide Mixture Induces Senescence In Normal Mesenchymal Stem Cells (MSC) And Promotes Tumorigenic Phenotype In Premalignant MSC - Archive ouverte HAL Access content directly
Journal Articles STEM CELLS Year : 2016

Low-Dose Pesticide Mixture Induces Senescence In Normal Mesenchymal Stem Cells (MSC) And Promotes Tumorigenic Phenotype In Premalignant MSC

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Abstract

Humans are chronically exposed to multiple environmental pollutants such as pesticides with no significant evidence about the safety of such poly-exposures. We exposed mesenchymal stem cells (MSC) to very low doses of mixture of seven pesticides frequently detected in food samples for 21 days in vitro. We observed a permanent phenotype modification with a specific induction of an oxidative stress-related senescence. Pesticide mixture also induced a shift in MSC differentiation towards adipogenesis but did not initiate a tumorigenic transformation. In modified MSC in which a premalignant phenotype wasinduced, the exposure to pesticide mixture promoted tumorigenic phenotype both in vitro andin vivo after cell implantation, in all nude mice. Our results suggest that a commoncombination of pesticides can induce a premature ageing of adult MSC, and as such couldaccelerate age-related diseases. Exposure to pesticide mixture may also promote thetumorigenic transformation in a predisposed stromal environment.
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Dates and versions

inserm-01398601 , version 1 (17-11-2016)

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  • HAL Id : inserm-01398601 , version 1

Cite

Mazène Hochane, Valérie Trichet, Claire Pecqueur, Pierre Avril, Lisa Oliver, et al.. Low-Dose Pesticide Mixture Induces Senescence In Normal Mesenchymal Stem Cells (MSC) And Promotes Tumorigenic Phenotype In Premalignant MSC. STEM CELLS, 2016. ⟨inserm-01398601⟩
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