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Journal Articles BMC Biotechnology Year : 2011

Proximity ligation in situ assay for monitoring the global DNA methylation in cells.

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Abstract

ABSTRACT: BACKGROUND: DNA methylation has a central role in the epigenetic control of mammalian gene expression, and is required for X inactivation, genomics imprinting and silencing of retrotransposons and repetitive sequences. Thus, several technologies have been developed to measure the degree of DNA methylation. RESULTS: We here present the development of the detection of protein-protein interactions via the adaptation of the proximity ligation in situ technology to evaluate the DNA methylation status in cells since the quantification of Dnmt1/PCNA interaction in cells reflects the degree of DNA methylation. CONCLUSION: This method being directly realizable on cells, it appears that it could suggest a wide range of applications in basic research and drug development. More particularly, this method is specially adapted for the investigations realized from a weak quantity of biologic materiel such as stem cells or primary cultured tumor cells for examples.
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Dates and versions

inserm-00587414 , version 1 (20-04-2011)

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Eric Hervouet, Philippe Hulin, François Vallette, Pierre-François Cartron. Proximity ligation in situ assay for monitoring the global DNA methylation in cells.. BMC Biotechnology, 2011, 11 (1), pp.31. ⟨10.1186/1472-6750-11-31⟩. ⟨inserm-00587414⟩
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