Interleukin-33 overexpression is associated with liver fibrosis in mice and humans. - Archive ouverte HAL Access content directly
Journal Articles Journal of Cellular and Molecular Medicine Year : 2010

Interleukin-33 overexpression is associated with liver fibrosis in mice and humans.

(1) , (1) , (1) , (1, 2) , (3, 4) , (5, 6) , (1) , (1) , (6) , (1) , (1)
1
2
3
4
5
6

Abstract

Interleukin-33 (IL-33), the most recently identified member of the IL-1 family, induces synthesis of T Helper 2 (Th2)-type cytokines via its heterodimeric ST2/IL-1RAcP receptor. Th2-type cytokines play an important role in fibrosis; thus, we investigated the role of IL-33 in liver fibrosis. IL-33, ST2 and IL-1RAcP gene expression was analysed in mouse and human normal (n= 6) and fibrotic livers (n= 28), and in human hepatocellular carcinoma (HCC; n= 22), using real-time PCR. IL-33 protein was detected in normal and fibrotic liver sections and in isolated liver cells using Western blotting and immunolocalization approaches. Our results showed that IL-33 and ST2 mRNA was overproduced in mouse and human fibrotic livers, but not in human HCC. IL-33 expression correlated with ST2 expression and also with collagen expression in fibrotic livers. The major sources of IL-33 in normal liver from both mice and human beings are the liver sinusoidal endothelial cells and, in fibrotic liver, the activated hepatic stellate cells (HSC). Moreover, IL-33 expression was increased in cultured HSC when stimulated by pro-inflammatory cytokines. In conclusion, IL-33 is strongly associated with fibrosis in chronic liver injury and activated HSC are a source of IL-33.

Dates and versions

hal-00660257 , version 1 (16-01-2012)

Identifiers

Cite

Pierrick Marvie, Mariette Lisbonne, Annie L'Helgoualc'H, Michel Rauch, Bruno Turlin, et al.. Interleukin-33 overexpression is associated with liver fibrosis in mice and humans.. Journal of Cellular and Molecular Medicine, 2010, 14 (6B), pp.1726-39. ⟨10.1111/j.1582-4934.2009.00801.x⟩. ⟨hal-00660257⟩
88 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More