Differential early response of monocyte/macrophage subsets to intra-operative corticosteroid administration in lung transplantation - Biologie de la reproduction, environnement, épigénétique et développement Access content directly
Journal Articles Frontiers in Immunology Year : 2023

Differential early response of monocyte/macrophage subsets to intra-operative corticosteroid administration in lung transplantation

Céline Urien
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Morgan Le Guen

Abstract

Methods: To address this issue, we used a cross-circulatory platform perfusing an extracorporeal lung coupled to cell mapping in the pig model, that enabled us to study the recruited cells in the allogeneic lung over 10 hours. Results: Myeloid cells, i.e. granulocytes and monocytic cells including classical CD14 pos and non-classical/intermediate CD16 pos cells, were the dominantly recruited subsets, with the latter upregulating the membrane expression of MHC class II and CD80/86 molecules. Whereas corticosteroids did not reduce the different cell subset recruitment, they potently dampened the MHC class II and CD80/86 expression on monocytic cells and not on alveolar macrophages. Besides, corticosteroids induced a temporary and partial anti-inflammatory gene profile depending on cytokines and monocyte/macrophage subsets. Discussion: This work documents the baseline effects of the standard-of-care corticosteroid treatment for early innate allo-responses. These insights will enable further optimization and improvement of lung transplantation outcomes.
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hal-04311493 , version 1 (28-11-2023)

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Matthieu Glorion, Florentina Pascale, Maxime Huriet, Jérôme Estephan, Carla Gouin, et al.. Differential early response of monocyte/macrophage subsets to intra-operative corticosteroid administration in lung transplantation. Frontiers in Immunology, 2023, 14, ⟨10.3389/fimmu.2023.1281546⟩. ⟨hal-04311493⟩
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