Study of the interaction between actinides and peptide ligands - Institut de Biosciences et Biotechnologies d'Aix-Marseille
Communication Dans Un Congrès Année : 2024

Study of the interaction between actinides and peptide ligands

Résumé

Actinides are radioactive metals of high importance in civil and military nuclear activities. Trace amounts of these elements may be found in the environment due to historical nuclear tests and accidental releases. Most actinides being alpha emitters, it is essential to investigate their behavior within the human body. While the targeted organs are well-known, the transport mechanism and molecular interactions remain unidentified. Proteins have been identified as actinide targets in the event of human contamination. Although the literature on the interactions between actinides and proteins is scarce, metalloproteomic studies have shown that several calcium-binding proteins have a strong affinity for actinides. As a model for all calciumbinding protein, the calcium-modulated protein (calmodulin) was chosen for its EF-hand motif site, which is widely present in calcium-biding proteins, and its high importance in cellular regulatory function. L. Daronnat investigated the interaction of plutonium (IV) with several variants of the calmodulin and observed various behaviors of the plutonium depending on the variant of calmodulin used2 : the formation of a Pu(IV)-calmodulin complex, the formation of a Pu(III)-calmodulin complex and the formation of hexanuclear clusters of plutonium. CaME (Engineered Calmodulin) is a variant of calmodulin developed especially to bind plutonium(IV). This variant has an extra carboxylate ligand in its complexation loop (shown in figure 1), increasing its affinity for actinides IV. This particular variant was the only which did not promote the formation of plutonium clusters. Instead, it only generated 1:1 complexes. Additionally, an unexpected change of the oxidation state of plutonium (from IV to III) was noticed in presence of CaME. To get a better understanding of this behavior, a deepened characterization of the species formed between CaME and actinides have been undertaken. This work aims to characterize the complexes formed between CaME and actinides (Th, U, Np, Pu), using different experimental techniques. Electrospray Ionization Mass Spectrometry was used to determine the stoichiometry of the complexes and UV-Vis-NIR Spectroscopy was used to characterize the environment of the actinide. Structural data were obtain through two or three-dimensional NMR spectroscopy. For that, an extra batch of CaME was synthetized and enriched with 13C and 15N, allowing for 1H-13C-15N NMR analysis. The paramagnetic properties of the plutonium are used to identify the amino acid involved in the coordination sphere of the metallic ions. (1) Aryal, B. P. J. Proteomics 2012, 75 (5), 1505–1514 (2) Daronnat, L. Doctorat Ph D thesis of the Montpellier University, 2023 (3) Daronnat, L. Inorg. Chem. 2023, 62 (21), 8334–8346
Fichier principal
Vignette du fichier
Study of the interaction between actinides and peptide ligands.pdf (295.02 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

cea-04736406 , version 1 (14-10-2024)

Identifiants

  • HAL Id : cea-04736406 , version 1

Citer

Emilien Faux, Loïc Daronnat, Catherine Berthomieu, Sandrine Sauge-Merle, Laurence Berthon-Nigond, et al.. Study of the interaction between actinides and peptide ligands. Atalante 2024, Sep 2024, AVIGNON, France. ⟨cea-04736406⟩
0 Consultations
0 Téléchargements

Partager

More